This study aimed to carry out complete ¹H and ¹³C NMR assignment of 13 protobassic acid saponins, including arganins A–C (1 –3 ) and F (4 ), butyrosides B–D (5 –7 ), tieghemelin (8 ), 3′‐O ‐glucosyl‐arganin C (9 ), Mi‐saponins A–C (10 –12 ), and mimusopsin (13 ), recorded in methanol‐d ₄. This was accomplished by the analysis of high‐resolution one‐dimensional (1D) NMR (¹H and ¹³C), two‐dimensional (2D) NMR (¹H–¹H COSY, HSQC, and HMBC), and selectively excited 1D TOCSY spectra. Before this study, ¹H and ¹³C NMR data of arganins A–C (1 –3 ) and F (4 ) were partially assigned. Our effort leads to their complete assignment, especially the glycon residue, and revises some reported data. Some revisions of the ¹H and ¹³C NMR data in the glycon part of butyroside C (6 ), tieghemelin (8 ), Mi‐saponin A (10 ), and mimusopsin (13 ) were made. Those data of butyrosides B and D (5 & 7 ) and Mi‐saponin B (11 ), which had not been recorded in methanol‐d ₄, are provided. In addition, the ¹H and ¹³C NMR data of Mi‐saponin C (12 ) are reported for the first time. These data, being recorded in methanol‐d ₄, should be more friendly for use as a reference for identifying the related triterpenoid saponins.

中文翻译：

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13种原碱性皂苷的1H和13C NMR全面归属

本研究旨在进行完整¹ H和¹³的13种protobassic酸皂甙，包括arganins A-C（C NMR分配13）和F（4），butyrosides B-d（5 - 7），tieghemelin（8） ，3'- Ô -glucosyl-arganin C（9），弥皂苷A-C（10 - 12），和mimusopsin（13），记录在甲醇- d ₄。这是通过分析高分辨率一维（1D）NMR（¹ H和¹³ C），二维（2D）NMR（¹ H– ¹ H COSY，HSQC和HMBC），并选择性激发一维TOCSY光谱。本研究之前，¹ H和^13个arganins的C NMR数据A-C（13）和F（4）被部分分配。我们的努力导致了它们的完全分配，尤其是糖苷残基，并修改了一些报道的数据。进行了丁糖苷C（6），tieghemelin（8），Mi- saponin A（10）和mimusopsin（13）糖苷部分¹ H和¹³ C NMR数据的一些修订。丁酯B和D的那些数据（5和7）和未在甲醇d _4中记录的Mi-皂苷B（11）。此外，Mi-皂苷C（12）的¹ H和¹³ C NMR数据是首次报道。这些记录在甲醇中的数据d ₄，应该更易于用作识别相关三萜皂苷的参考。