Controlled aggregation of amphiphilic aggregation‐induced emission polycation and superparamagnetic iron oxide nanoparticles as fluorescence/magnetic resonance imaging probes,Journal of Applied Polymer Science

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Controlled aggregation of amphiphilic aggregation‐induced emission polycation and superparamagnetic iron oxide nanoparticles as fluorescence/magnetic resonance imaging probes
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2019-11-30 , DOI: 10.1002/app.48760
Shengxiang Fu ₁ , Zhongyuan Cai ₁ , Li Liu ₁ , Li Yang ₁ , Rongrong Jin ₁ , Zhiyun Lu ₂ , Hua Ai _{1,

3}

Affiliation

Fluorescence/magnetic resonance (MR) dual‐mode imaging, which combines the excellent single‐cell sensitivity of fluorescence imaging and the high spatial resolution of MR imaging, has been applied to different biomedical applications. However, the aggregation‐caused quenching characteristic of most fluorescence molecules often put limits in their applications. Herein, a fluorescence/MR dual‐mode imaging probe [polyethylene glycol‐polyethylenimine‐tetraphenylethene (PEG‐PEI‐TPE)/superparamagnetic iron oxide (SPIO)] with aggregation‐induced emission characteristic is prepared by coupling poly(acrylic acid)‐coated SPIO with PEG‐PEI‐TPE. The fluorescence intensity and lifetime of PEG‐PEI‐TPE/SPIO is higher than PEG‐PEI‐TPE especially at lower polymer concentrations (≤0.2 mg mL⁻¹). Moreover, the fluorescence intensity of PEG‐PEI‐TPE/SPIO gradually increased along with the decline of the pH from 9.0 to 4.0, which is beneficial for studying intracellular organelles. The T₂ relaxivity of PEG‐PEI‐TPE/SPIO is 212.3 Fe mM⁻¹ s⁻¹ under a 3.0 T MR scanner. Cell labeling experiment shows that PEG‐PEI‐TPE/SPIO can effectively label RAW 264.7 and Hela cells, and labeled cells are visible under both fluorescence and clinical MR examinations. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48760.

中文翻译：

作为荧光/磁共振成像探针的两亲性聚集诱导的发射聚阳离子和超顺磁性氧化铁纳米粒子的受控聚集

结合了出色的荧光成像单细胞灵敏度和MR成像的高空间分辨率的荧光/磁共振（MR）双模成像技术已应用于不同的生物医学应用中。但是，大多数荧光分子的聚集引起的猝灭特性通常会限制其应用。本文中，通过偶联聚丙烯酸涂层制备了具有聚集诱导发射特性的荧光/ MR双模成像探针[聚乙二醇-聚乙烯亚胺-四苯基乙烯（PEG-PEI-TPE）/超顺磁性氧化铁（SPIO）]。带有PEG‐PEI‐TPE的SPIO。PEG‐PEI‐TPE / SPIO的荧光强度和寿命高于PEG‐PEI‐TPE，尤其是在较低的聚合物浓度下（≤0.2mg mL ^-1）。此外，随着pH从9.0降低到4.0，PEG-PEI-TPE / SPIO的荧光强度逐渐增加，这对于研究细胞内细胞器是有益的。在3.0 T MR扫描仪下，PEG-PEI-TPE / SPIO的T ₂弛豫度为212.3 Fe m M ^-1 s ^-1。细胞标记实验表明，PEG-PEI-TPE / SPIO可以有效标记RAW 264.7和Hela细胞，并且在荧光检查和临床MR检查下可见被标记的细胞。分级为4 +©2019 Wiley Periodicals，Inc.J.Appl。Polym。科学 2020，137，48760。

更新日期：2020-03-26

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