Acoustic Holographic Cell Patterning in a Biocompatible Hydrogel.,Advanced Materials

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Acoustic Holographic Cell Patterning in a Biocompatible Hydrogel.
Advanced Materials ( IF 27.4 ) Pub Date : 2019-11-29 , DOI: 10.1002/adma.201904181
Zhichao Ma ₁ , Andrew W Holle ₂ , Kai Melde ₁ , Tian Qiu _{1,

3} , Korbinian Poeppel ₁ , Vincent Mauricio Kadiri _{1,

3} , Peer Fischer _{1,

3}

Affiliation

Acoustophoresis is promising as a rapid, biocompatible, noncontact cell manipulation method, where cells are arranged along the nodes or antinodes of the acoustic field. Typically, the acoustic field is formed in a resonator, which results in highly symmetric regular patterns. However, arbitrary, nonsymmetrically shaped cell assemblies are necessary to obtain the irregular cellular arrangements found in biological tissues. It is shown that arbitrarily shaped cell patterns can be obtained from the complex acoustic field distribution defined by an acoustic hologram. Attenuation of the sound field induces localized acoustic streaming and the resultant convection flow gently delivers the suspended cells to the image plane where they form the designed pattern. It is shown that the process can be implemented in a biocompatible collagen solution, which can then undergo gelation to immobilize the cell pattern inside the viscoelastic matrix. The patterned cells exhibit F-actin-based protrusions, which indicate that the cells grow and thrive within the matrix. Cell viability assays and brightfield imaging after one week confirm cell survival and that the patterns persist. Acoustophoretic cell manipulation by holographic fields thus holds promise for noncontact, long-range, long-term cellular pattern formation, with a wide variety of potential applications in tissue engineering and mechanobiology.

中文翻译：

生物相容性水凝胶中的声全息细胞图案。

声电疗法有望作为一种快速，生物相容的非接触式细胞操作方法，其中细胞沿着声场的波节或波腹排列。通常，声场在谐振器中形成，这导致高度对称的规则图案。但是，为了获得生物组织中不规则的细胞排列，必须使用任意形状不对称的细胞组件。示出了可以从由声全息图定义的复声场分布获得任意形状的单元图案。声场的衰减引起局部声流，并且所产生的对流将悬浮的细胞缓慢地传递至图像平面，在此处它们形成设计的图案。结果表明，该过程可以在生物相容性胶原蛋白溶液中实施，然后可以进行凝胶化以固定粘弹性基质内部的细胞模式。图案化的细胞显示出基于F-肌动蛋白的突起，这表明细胞在基质中生长并繁盛。一周后进行细胞活力测定和明场成像，确认细胞存活并且模式持续存在。因此，通过全息领域的声电泳细胞操纵有望实现非接触，长期，长期的细胞模式形成，在组织工程和机械生物学中具有广泛的潜在应用。一周后进行细胞活力测定和明场成像，确认了细胞存活并且这种模式持续存在。因此，通过全息领域的声电泳细胞操纵有望实现非接触，长期，长期的细胞模式形成，在组织工程和机械生物学中具有广泛的潜在应用。一周后进行细胞活力测定和明场成像，确认了细胞存活并且这种模式持续存在。因此，通过全息领域的声电泳细胞操纵有望实现非接触，长期，长期的细胞模式形成，在组织工程和机械生物学中具有广泛的潜在应用。

更新日期：2020-01-27

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