Development of Bis-GMA-free biopolymer to avoid estrogenicity.,Dental Materials

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Development of Bis-GMA-free biopolymer to avoid estrogenicity.
Dental Materials ( IF 4.6 ) Pub Date : 2019-11-30 , DOI: 10.1016/j.dental.2019.11.016
Soo-Kyung Jun ₁ , Jae-Ryung Cha ₂ , Jonathan C Knowles ₃ , Hae-Won Kim ₄ , Jung-Hwan Lee ₄ , Hae-Hyoung Lee ₅

Affiliation

Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Dental Hygiene, Kyungdong University, Wonju 26495, South Korea.
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Research Center, Dankook University, Cheonan 330-714, South Korea.
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Research Center, Dankook University, Cheonan 330-714, South Korea; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan 31116, South Korea; Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, London, UK; The Discoveries Centre for Regenerative and Precision Medicine, Eastman Dental Institute, University College London, London, UK.
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Research Center, Dankook University, Cheonan 330-714, South Korea; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan 31116, South Korea.
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea.

OBJECTIVE Although bisphenol A-glycidyl methacrylate (Bis-GMA)-based dental materials are widely used in dentistry, Estrogenicity from released bisphenol A remains a concern due to possibility of adversely affecting the growth of children and homeostasis of adults. Here, a new family of isosorbide-derived biomonomers were synthesized and experimentally utilized as a matrix of dental sealants to provide physico-mechanical and biological properties comparable to those of a conventional Bis-GMA-based material but without the the potential estrogenicity. METHODS After synthesis of isosorbide-derived biomonomers (ISDB) by light polymerization, an experimental dental sealant with different silica filler concentrations (0-15wt%) was characterized and compared to a commercially available Bis-GMA-based sealant. Cytotoxicity and estrogenicity assays were conducted with human oral keratinocytes and estrogen-sensitive MCF-7 cells, respectively. RESULTS ISDB-based dental sealants exhibited typical initially smooth surfaces with depth of cure, Vickers hardness, compressive strength/modulus, water resorption/solubility, and flowability comparable to those of the commercial sealant and met the ISO standard for dental sealants and polymer-based restorative materials. Indirect cytotoxicity tests using an extract showed comparable viability among experimental ISDB-based materials and a commercial Bis-GMA-incorporated control. DNA synthesis in MCF-7 cells (a marker of estrogenicity) and the release of bisphenol A under enzymatic incubation were not detected in ISDB-based materials. SIGNIFICANCE In conclusion, the comparable physico-mechanical properties of ISDB-based materials with their cytocompatibility and lack of estrogenicity suggest the potential usefulness of ISDBs as a newly developed and safe biomaterial.

中文翻译：

开发不含Bis-GMA的生物聚合物，以避免雌激素。

目的尽管基于双酚A-甲基丙烯酸缩水甘油酯（Bis-GMA）的牙科材料已广泛用于牙科领域，但由于释放出的双酚A的雌激素性可能会不利地影响儿童的成长和成人的体内平衡，因此仍然值得关注。在这里，合成了一个新的异山梨醇衍生的生物单体家族，并将其作为牙科密封胶的基质进行实验，以提供与传统的基于Bis-GMA的材料相媲美的物理机械和生物学特性，但没有潜在的雌激素作用。方法通过光聚合合成异山梨醇衍生的生物单体（ISDB）之后，表征了具有不同二氧化硅填料浓度（0-15wt％）的实验性牙科密封剂，并将其与市售的基于Bis-GMA的密封剂进行了比较。分别使用人口腔角质形成细胞和雌激素敏感性MCF-7细胞进行了细胞毒性和雌激素测定。结果基于ISDB的牙科密封剂表现出典型的初始光滑表面，其固化深度，维氏硬度，抗压强度/模量，吸水率/溶解性和流动性与商业密封剂相当，并且符合牙科密封剂和聚合物基的ISO标准修复材料。使用提取物的间接细胞毒性测试显示，在基于实验的基于ISDB的材料和与Bis-GMA结合的商业对照中，具有同等的可行性。在基于ISDB的材料中未检测到MCF-7细胞中的DNA合成（雌激素性的标记）和酶促孵育下双酚A的释放。意义总之，

更新日期：2019-11-30

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