Circular RNA (circRNA) is important in the pathogenesis of many diseases. By analyzing the GSE96964 microarray, hsa_circ_0000285 (circ-0000285) was found to be highly expressed in osteosarcoma. Recent studies have shown that circ-0000285 is capable of regulating proliferative and migratory potentials. Here, we investigated the potential functions in regulating osteosarcoma cells to proliferate and migrate. First of all, qRT-PCR data revealed a higher level of circ-0000285 in osteosarcoma cell lines relative to normal osteoblasts. Through dual-luciferase reporter gene assay and RIP assay, we confirmed that both circ-0000285 and TGFB2 could directly bind to miRNA-599. Regulatory effects of circ-0000285 and miRNA-599 on proliferative and migratory potentials were evaluated by EdU assay and transwell migration assay. It is indicated that circ-0000285 overexpression enhanced the proliferative and migratory potentials of osteosarcoma, which could be reversed by miRNA-599 overexpression. This study revealed a vital role of circ-0000285/miRNA-599/TGFB2 axis in regulating the progression of osteosarcoma, providing a novel perspective for clarifying its pathogenesis.

环状RNA（circRNA）在许多疾病的发病机制中很重要。通过分析 GSE96964 微阵列，发现 hsa_circ_0000285 (circ-0000285) 在骨肉瘤中高表达。最近的研究表明，circ-0000285 能够调节增殖和迁移潜能。在这里，我们研究了调节骨肉瘤细胞增殖和迁移的潜在功能。首先，qRT-PCR 数据显示，相对于正常成骨细胞，骨肉瘤细胞系中的 circ-0000285 水平更高。通过双荧光素酶报告基因检测和RIP检测，我们证实circ-0000285和TGFB2都可以直接与miRNA-599结合。通过 EdU 测定和 transwell 迁移测定评估 circ-0000285 和 miRNA-599 对增殖和迁移潜力的调节作用。表明circ-0000285过表达增强了骨肉瘤的增殖和迁移潜能，这可以通过miRNA-599过表达来逆转。该研究揭示了circ-0000285/miRNA-599/TGFB2轴在调节骨肉瘤进展中的重要作用，为阐明其发病机制提供了新的视角。