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Microwave-assisted preparation of ZnFe2O4-Ag/rGO nanocomposites for amplification signal detection of alpha-fetoprotein.
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2019-11-30 , DOI: 10.1016/j.bioelechem.2019.107434 Mei Wu 1 , Yuxiao Yang 1 , Kaihang Cao 1 , Chulei Zhao 1 , Xiuwen Qiao 1 , Chenglin Hong 1
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2019-11-30 , DOI: 10.1016/j.bioelechem.2019.107434 Mei Wu 1 , Yuxiao Yang 1 , Kaihang Cao 1 , Chulei Zhao 1 , Xiuwen Qiao 1 , Chenglin Hong 1
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In this study, a novel signal-amplified immunosensor was designed by using a microwave-assisted self-assembly method to synthesize ZnFe2O4-Ag/rGO nanocomposites. The conductivity of ZnFe2O4-rGO nanocomposites was significantly improved due to the effective inhibition of rGO accumulation by the insertion of ZnFe2O4 and Ag nanoparticles (NPs) into graphene sheets. Excellent sensitivity and reproducibility were achieved through the microwave-assisted preparation of ZnFe2O4-Ag/rGO nanocomposites as a substrate, with the Ag NPs enhancing the signal because of the effective conductive matrix. The layer assembly process of the immunosensor was verified by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimal conditions, the fabricated immunosensor showed good linearity over a wide concentration range from 1 pg mL-1 to 200 ng mL-1 with a low detection limit of 0.98 pg mL-1, and exhibited excellent specificity, good stability, and reproducibility. These qualities can contribute to the successful application of a label-free immunosensor in the detection of AFP in human serum.
中文翻译：
微波辅助制备ZnFe2O4-Ag / rGO纳米复合材料，用于放大甲胎蛋白的信号检测。
在这项研究中，通过使用微波辅助自组装方法设计了一种新型的信号放大免疫传感器，以合成ZnFe2O4-Ag / rGO纳米复合材料。ZnFe2O4-rGO纳米复合材料的电导率显着提高，这是由于通过将ZnFe2O4和Ag纳米颗粒（NPs）插入石墨烯片材而有效抑制了rGO积累。通过微波辅助制备ZnFe2O4-Ag / rGO纳米复合材料作为基质，由于有效的导电基质，Ag NPs增强了信号，从而获得了出色的灵敏度和可重复性。通过循环伏安法和电化学阻抗谱验证了免疫传感器的层组装过程。在最佳条件下 所制备的免疫传感器在1 pg mL-1至200 ng mL-1的宽浓度范围内均显示出良好的线性，检出限低至0.98 pg mL-1，并具有出色的特异性，稳定性和可重复性。这些品质可有助于无标记免疫传感器在人类血清中AFP检测中的成功应用。
更新日期：2019-11-30
中文翻译：
微波辅助制备ZnFe2O4-Ag / rGO纳米复合材料，用于放大甲胎蛋白的信号检测。
在这项研究中，通过使用微波辅助自组装方法设计了一种新型的信号放大免疫传感器，以合成ZnFe2O4-Ag / rGO纳米复合材料。ZnFe2O4-rGO纳米复合材料的电导率显着提高，这是由于通过将ZnFe2O4和Ag纳米颗粒（NPs）插入石墨烯片材而有效抑制了rGO积累。通过微波辅助制备ZnFe2O4-Ag / rGO纳米复合材料作为基质，由于有效的导电基质，Ag NPs增强了信号，从而获得了出色的灵敏度和可重复性。通过循环伏安法和电化学阻抗谱验证了免疫传感器的层组装过程。在最佳条件下 所制备的免疫传感器在1 pg mL-1至200 ng mL-1的宽浓度范围内均显示出良好的线性，检出限低至0.98 pg mL-1，并具有出色的特异性，稳定性和可重复性。这些品质可有助于无标记免疫传感器在人类血清中AFP检测中的成功应用。
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