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Employment of electrostriction phenomenon for label-free electrochemical immunosensing of tetracycline.
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2019-11-29 , DOI: 10.1016/j.bioelechem.2019.107405 Karolina Starzec 1 , Cecilia Cristea 2 , Mihaela Tertis 2 , Bogdan Feier 2 , Marcin Wieczorek 1 , Paweł Kościelniak 1 , Jolanta Kochana 1
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2019-11-29 , DOI: 10.1016/j.bioelechem.2019.107405 Karolina Starzec 1 , Cecilia Cristea 2 , Mihaela Tertis 2 , Bogdan Feier 2 , Marcin Wieczorek 1 , Paweł Kościelniak 1 , Jolanta Kochana 1
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The presented work describes a simple label-free electrochemical immunosensor for the determination of tetracycline (TC). The functioning of the sensor was based on the electrostriction of a antibody-terminated thiol layer self-assembled on a gold electrode surface, serving as a dielectric membrane. The intensity of electrostriction was correlated with the amount of TC captured through an affinity reaction with its specific antibody, and was followed in the form of capacitance-potential curves. The process of the immunosensor construction was optimized using electrochemical impedance spectroscopy (EIS). The chemisorption time of the thiol, the duration of the TCAb immobilization and its concentration were optimized. The developed immunosensor exhibited a linear response in two concentration ranges: from 0.95 to 10 μmol L-1 and from 10 to 140 μmol L-1, with the mean sensitivity of 6.27 nF μmol-1 L (88.67 nF μmol-1 L cm-2) and 0.56 nF μmol-1 L (7.84 nF μmol-1 L cm-2), respectively. The limit of detection was evaluated as 28 nmol L-1. The specificity of the proposed sensor toward other antibiotics, amoxicillin and ciprofloxacin, was examined. The immunosensor was successfully employed to quantify TC in a tablet form and in a matrix of river water.
中文翻译：
使用电致伸缩现象进行四环素无标签电化学免疫传感。
提出的工作描述了一种简单的无标记电化学免疫传感器，用于测定四环素（TC）。传感器的功能是基于在电电极膜上自组装在金电极表面上自组装的抗体封端的硫醇层的电致伸缩。电致伸缩的强度与通过与特异性抗体的亲和反应捕获的TC量相关，并以电容-电位曲线的形式跟踪。免疫传感器的构建过程使用电化学阻抗谱（EIS）进行了优化。优化了硫醇的化学吸附时间，TCAb固定的持续时间及其浓度。开发的免疫传感器在两个浓度范围内显示线性响应：从0开始。95至10μmolL-1和10至140μmolL-1，平均灵敏度为6.27 nFμmol-1L（88.67 nFμmol-1L cm-2）和0.56 nFμmol-1L（7.84 nFμmol -1 L cm-2）。检测极限被评价为28nmol L-1。检查了拟议的传感器对其他抗生素阿莫西林和环丙沙星的特异性。免疫传感器已成功用于定量片剂形式和河水基质中的TC。
更新日期：2019-11-30
中文翻译：
使用电致伸缩现象进行四环素无标签电化学免疫传感。
提出的工作描述了一种简单的无标记电化学免疫传感器，用于测定四环素（TC）。传感器的功能是基于在电电极膜上自组装在金电极表面上自组装的抗体封端的硫醇层的电致伸缩。电致伸缩的强度与通过与特异性抗体的亲和反应捕获的TC量相关，并以电容-电位曲线的形式跟踪。免疫传感器的构建过程使用电化学阻抗谱（EIS）进行了优化。优化了硫醇的化学吸附时间，TCAb固定的持续时间及其浓度。开发的免疫传感器在两个浓度范围内显示线性响应：从0开始。95至10μmolL-1和10至140μmolL-1，平均灵敏度为6.27 nFμmol-1L（88.67 nFμmol-1L cm-2）和0.56 nFμmol-1L（7.84 nFμmol -1 L cm-2）。检测极限被评价为28nmol L-1。检查了拟议的传感器对其他抗生素阿莫西林和环丙沙星的特异性。免疫传感器已成功用于定量片剂形式和河水基质中的TC。
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