Malathion (MAL) is an organophosphorus (OP) insecticide. It is a cholinesterase inhibitor, which can pose serious health and environmental problems. In this study, a sensitive and selective molecular imprinted polymer (MIP) based on screen-printed gold electrodes (Au-SPE) for MAL detection in olive oils and fruits, was devised. The MIP sensor was prepared using acrylamide as the functional monomer and MAL as the template. Subsequently, the morphology of the electrode surface was studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electrochemical characterization of the developed MIP sensor was performed by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The operational repeatability and stability of the sensor were studied. It was found to have a dynamic concentration range of (0.1 pg mL-1-1000 pg mL-1) and a low limit of detection (LOD) of 0.06 pg mL-1. Furthermore, the sensor was employed to determine MAL content in olive oil with a recovery rate of 87.9% and a relative standard deviation of 8%. It was successfully applied for MAL determination in real samples and promise to open new opportunities for the detection of OP pesticides residues in various food products, as well as in environmental applications.

中文翻译：

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分子印迹聚合物电化学传感器的开发及其在灵敏检测和测定橄榄果和油中马拉硫磷中的应用。

马拉硫磷（MAL）是一种有机磷（OP）杀虫剂。它是胆碱酯酶抑制剂，可能造成严重的健康和环境问题。在这项研究中，设计了一种基于丝网印刷金电极（Au-SPE）的灵敏且选择性的分子印迹聚合物（MIP），用于橄榄油和水果中的MAL检测。MIP传感器使用丙烯酰胺作为功能单体，MAL作为模板制备。随后，通过扫描电子显微镜（SEM）和原子力显微镜（AFM）研究了电极表面的形貌。通过循环伏安法（CV），差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）技术对开发的MIP传感器进行电化学表征。研究了传感器的操作重复性和稳定性。发现其动态浓度范围为（0.1 pg mL-1-1000 pg mL-1），检测下限（LOD）为0.06 pg mL-1。此外，该传感器用于测定橄榄油中的MAL含量，回收率为87.9％，相对标准偏差为8％。它已成功用于实际样品中的MAL测定，并有望为检测各种食品中以及环境应用中的OP农药残留提供新的机会。