The protective effect of thymoquinone on tert-butylhydroquinone induced cytotoxicity in human umbilical vein endothelial cells.,Toxicology Research

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The protective effect of thymoquinone on tert-butylhydroquinone induced cytotoxicity in human umbilical vein endothelial cells.
Toxicology Research ( IF 2.2 ) Pub Date : 2019-11-29 , DOI: 10.1039/c9tx00235a
Zahra Karimi _{1,

2,

3} , Maryam Ghaffari ₁ , Jafar Ezzati Nazhad Dolatabadi ₄ , Parvin Dehghan ₂

Affiliation

2-tert-Butyl-4-hydroquinone (TBHQ) is used for inhibition of oxidative rancidity in the food industry. However, this antioxidant can stimulate cytotoxicity in human umbilical vein endothelial cells (HUVECs). Thus, potential protective effects of thymoquinone (TQ) against TBHQ-induced cytotoxicity were investigated. Cytotoxicity was evaluated via MTT, flow cytometry, DAPI staining and DNA fragmentation methods. The obtained results revealed that treatment of HUVECs with TQ enhanced the cell viability rate and it had potential to reduce the cytotoxicity effect of TBHQ in cells. Also, in a combined regime of TQ and TBHQ, apoptosis was reduced compared to the cells treated with TBHQ (p < 0.05). Similarly, TQ had a protective effect on DNA and chromatin fragmentation of the cells treated with TBHQ. Finally, it can be concluded that TQ could be used as a protective agent against cytotoxicity induced by TBHQ in HUVECs.

中文翻译：

百里醌对叔丁基氢醌诱导的人脐静脉内皮细胞细胞毒性的保护作用。

2-叔丁基-4-氢醌 (TBHQ) 用于抑制食品工业中的氧化酸败。然而，这种抗氧化剂可以刺激人脐静脉内皮细胞（HUVEC）的细胞毒性。因此，研究了百里醌（TQ）对 TBHQ 诱导的细胞毒性的潜在保护作用。通过MTT、流式细胞术、DAPI染色和DNA片段化方法评估细胞毒性。所得结果表明，用TQ处理HUVEC可提高细胞存活率，并有可能降低TBHQ对细胞的细胞毒性作用。此外，在 TQ 和 TBHQ 的联合治疗方案中，与用 TBHQ 处理的细胞相比，细胞凋亡减少 ( p < 0.05)。同样，TQ 对 TBHQ 处理的细胞的 DNA 和染色质碎片具有保护作用。最后，可以得出结论，TQ 可用作 HUVEC 中 TBHQ 诱导的细胞毒性的保护剂。

更新日期：2019-11-29

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