Elevated intraocular pressure (IOP) is the primary risk factor for glaucoma and is the only treatable feature of the disease. There is a correlation between elevated pressure and homeostatic reductions in the aqueous humor outflow resistance via changes in the extracellular matrix of the trabecular meshwork. It is unclear how these extracellular matrix changes affect segmental patterns of aqueous humor outflow, nor do we understand their causal relationship. The goal of this study was to determine whether there are changes in the segmental outflow regions with perfusion in normal eyes, and whether these regions change during the IOP homeostatic response to elevated pressure. Using human anterior segment perfusion organ culture, we measured the amount of high flow (HF), intermediate flow (MF), and low flow (LF) regions before and after 7 days of perfusion at either physiologic pressure ("1x") or at elevated pressure ("2x"). We found a small but significant decrease in the amount of HF regions over 7 days perfusion at 1x pressure, and a twofold increase in the amount of MF regions over 7 days perfusion at 2x pressure. Small positional differences, or shifts in the specific location of HF, MF, or LF, occurred on a per eye basis and were not found to be statistically significant across biological replicates. Differences in the amount of segmental flow regions of contralateral eyes flowed at 1x pressure for 7 days were small and not statistically significant. These results demonstrate that perfusion at physiologic pressure had little effect on the distribution and amount of HF, MF and LF regions. However, the overall amount of MF regions is significantly increased in response to perfusion at elevated pressure during IOP homeostatic resistance adjustment. The amount of both HF and LF regions was decreased accordingly suggesting a coordinated response in the TM to elevated pressure.

中文翻译：

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压力升高会影响小梁网段区域的相对分布。

眼内压升高（IOP）是青光眼的主要危险因素，并且是该病的唯一可治疗特征。通过小梁网的细胞外基质的改变，升高的压力与房水流出阻力的体内稳态降低之间存在相关性。目前尚不清楚这些细胞外基质的变化如何影响房水流出的节段模式，我们也不清楚它们的因果关系。这项研究的目的是确定正常眼中灌注引起的节段性流出区域是否发生变化，以及这些区域在眼压对压力升高的体内稳态反应过程中是否发生变化。使用人类前节灌注器官培养，我们测量了高流量（HF），中流量（MF），和在生理压力（“ 1x”）或高压（“ 2x”）灌注7天之前和之后的低流量（LF）区域。我们发现在1倍压力下灌注7天后HF区域的数量略有减少，但显着降低，而在2倍压力下灌注7天后MF区域的数量增加了两倍。细微的位置差异，或HF，MF或LF特定位置的移位，是每只眼睛发生的，在生物学复制中未发现统计学上的显着差异。对侧眼的节段性流区域在1x压力下流动7天的流量差异很小，且无统计学意义。这些结果表明，在生理压力下的灌注对HF，MF和LF区域的分布和数量几乎没有影响。然而，在进行IOP稳态阻力调整时，由于高压下的灌注，MF区域的总量显着增加。HF和LF区域的数量相应减少，这表明TM对升高的压力具有协调的响应。