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Identification of the biliary selenium metabolite and the biological significance of selenium enterohepatic circulation.
Metallomics ( IF 2.9 ) Pub Date : 2019-12-06 , DOI: 10.1039/c9mt00274j Kazuaki Takahashi 1 , Yasumitsu Ogra
Metallomics ( IF 2.9 ) Pub Date : 2019-12-06 , DOI: 10.1039/c9mt00274j Kazuaki Takahashi 1 , Yasumitsu Ogra
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Although selenium (Se) is mainly excreted in urine, it has been reported that an unknown Se metabolite is excreted in bile. When we administered selenomethionine (SeMet), selenocyanate or selenite to rats, a common biliary selenometabolite was detected 10 min after administration. The amount of the selenometabolite originating from SeMet was less than that originating from the two inorganic Se compounds, selenocyanate and selenite, suggesting that the transformation from the methylated organic selenocompound, i.e., SeMet, was less efficient than that from the inorganic Se compounds. The common biliary selenometabolite was concretely identified as selenodiglutathione (GSSeSG) by two types of mass spectrometry, i.e., LC-inductively coupled mass spectrometry (ICP-MS) and LC-ESI-Q/TOF. The bile-drained rats had lower urinary Se levels than the sham-operated rats. In addition, the Se amounts in urine plus bile of the bile-drained rats were comparable to the Se amount in the urine of the sham-operated rats. These results suggest that the biliary selenometabolite, GSSeSG, was reabsorbed in the gut and finally excreted in urine. Enterohepatic circulation occurs to maintain Se status in the body.
中文翻译：
胆汁中硒代谢物的鉴定及其硒肝肠循环的生物学意义。
尽管硒（Se）主要从尿中排泄，但是据报道，未知的硒代谢物在胆汁中排泄。当我们给大鼠施用硒代蛋氨酸（SeMet），硒代氰酸酯或亚硒酸盐时，在给药10分钟后发现了常见的胆源性硒代代谢产物。源自SeMet的硒代代谢物的量小于源自两种无机Se化合物的硒代氰酸盐和亚硒酸盐的量，表明从甲基化的有机硒化合物即SeMet的转化效率低于从无机Se化合物的转化。通过两种质谱法，即LC电感耦合质谱法（ICP-MS）和LC-ESI-Q / TOF，将胆总硒代代谢物具体确定为硒二谷胱甘肽（GSSeSG）。胆汁引流的大鼠的尿中硒水平低于假手术的大鼠。此外，胆汁引流大鼠尿液和胆汁中的硒含量与假手术大鼠尿液中的硒含量相当。这些结果表明胆脂硒代谢产物GSSeSG在肠道中被重新吸收，并最终在尿液中排泄。发生肝肠循环以维持体内硒状态。
更新日期：2020-02-27
中文翻译：
胆汁中硒代谢物的鉴定及其硒肝肠循环的生物学意义。
尽管硒（Se）主要从尿中排泄，但是据报道，未知的硒代谢物在胆汁中排泄。当我们给大鼠施用硒代蛋氨酸（SeMet），硒代氰酸酯或亚硒酸盐时，在给药10分钟后发现了常见的胆源性硒代代谢产物。源自SeMet的硒代代谢物的量小于源自两种无机Se化合物的硒代氰酸盐和亚硒酸盐的量，表明从甲基化的有机硒化合物即SeMet的转化效率低于从无机Se化合物的转化。通过两种质谱法，即LC电感耦合质谱法（ICP-MS）和LC-ESI-Q / TOF，将胆总硒代代谢物具体确定为硒二谷胱甘肽（GSSeSG）。胆汁引流的大鼠的尿中硒水平低于假手术的大鼠。此外，胆汁引流大鼠尿液和胆汁中的硒含量与假手术大鼠尿液中的硒含量相当。这些结果表明胆脂硒代谢产物GSSeSG在肠道中被重新吸收，并最终在尿液中排泄。发生肝肠循环以维持体内硒状态。
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