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LC-MSMS based screening of emerging pollutant degradation by different peroxidases.
BMC Biotechnology ( IF 3.5 ) Pub Date : 2019-11-28 , DOI: 10.1186/s12896-019-0574-y
Khadega A Almaqdi 1 , Rana Morsi 1 , Bahia Alhayuti 1 , Farah Alharthi 1 , S Salman Ashraf 2
Affiliation  

BACKGROUND The presence of a wide range of bioactive organic pollutants in wastewater and municipal water sources is raising concerns about their potential effects on humans. Not surprisingly, various approaches are being explored that can efficiently degrade these persistent organic pollutants. Use of peroxidases has recently been recognized as a novel remediation approach that may have potential advantages over conventional degradation techniques. However, testing the abilities of different peroxidases to degrade diverse emerging pollutants is tedious and cumbersome. RESULTS In the present study, we present a rapid and robust approach to easily test the degradability of 21 different emerging pollutants by five different peroxidases (soybean peroxidase, chloroperoxidase, lactoperoxidase, manganese peroxidase, and horseradish peroxidase) using an LC-MSMS approach. Furthermore, this approach was also used to examine the role of a redox mediator in these enzymatic degradation assays. Our results show that some of the organic pollutants can be easily degraded by all five of the peroxidases tested, whereas others are only degraded by a specific peroxidase (or when a redox mediator was present) and there are some that are completely resistant to degradation by any of the peroxidases tested (even in the presence of a redox mediator). The degradation of furosemide and trimethoprim by soybean peroxidase and chloroperoxidase, respectively, was investigated in detail by examining the transformation products generated during their degradation. Some of the products generated during enzymatic breakdown of these pollutants have been previously reported by others, however, we report many new transformation products. CONCLUSIONS LC-MSMS approaches, like the one described here, can be used to rapidly evaluate the potential of different peroxidases (and redox requirements) to be used as bioremediation agents. Our preliminary result shows peroxidases hold tremendous potential for being used in a final wastewater treatment step.

中文翻译:


基于 LC-MSMS 的不同过氧化物酶降解新兴污染物的筛选。



背景技术废水和城市水源中存在多种生物活性有机污染物,引起了人们对其对人类潜在影响的担忧。毫不奇怪,人们正在探索各种可以有效降解这些持久性有机污染物的方法。过氧化物酶的使用最近被认为是一种新颖的修复方法,与传统的降解技术相比可能具有潜在的优势。然而,测试不同过氧化物酶降解各种新兴污染物的能力是乏味且麻烦的。结果在本研究中,我们提出了一种快速而稳健的方法,使用 LC-MSMS 方法轻松测试 5 种不同过氧化物酶(大豆过氧化物酶、氯过氧化物酶、乳过氧化物酶、锰过氧化物酶和辣根过氧化物酶)对 21 种不同新兴污染物的降解能力。此外,该方法还用于检查氧化还原介体在这些酶促降解测定中的作用。我们的结果表明,一些有机污染物可以很容易地被所有五种测试的过氧化物酶降解,而另一些有机污染物只能被特定的过氧化物酶(或存在氧化还原介体时)降解,并且有些有机污染物完全抵抗过氧化酶的降解。任何测试的过氧化物酶(即使存在氧化还原介体)。通过检查降解过程中产生的转化产物,详细研究了大豆过氧化物酶和氯过氧化物酶分别对呋塞米和甲氧苄啶的降解。这些污染物的酶分解过程中产生的一些产物之前已被其他人报道过,但是,我们报告了许多新的转化产物。 结论 LC-MSMS 方法(如此处描述的方法)可用于快速评估不同过氧化物酶(和氧化还原要求)用作生物修复剂的潜力。我们的初步结果表明,过氧化物酶在最终废水处理步骤中具有巨大的潜力。
更新日期:2020-04-22
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