Cell-free gene expression: an expanded repertoire of applications.,Nature Reviews Genetics

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Cell-free gene expression: an expanded repertoire of applications.
Nature Reviews Genetics ( IF 39.1 ) Pub Date : 2019-11-28 , DOI: 10.1038/s41576-019-0186-3
Adam D Silverman _{1,

2,

3} , Ashty S Karim _{1,

2,

3} , Michael C Jewett _{1,

2,

3,

4,

5,

6}

Affiliation

Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.

中文翻译：

无细胞基因表达：扩展的应用范围。

无细胞生物学是在不使用完整活细胞的情况下激活生物过程。它已在生命科学中用作基础研究工具已有50多年的历史，但最近的技术复兴促进了高产（每升蛋白质的克数），模型细菌的无细胞基因表达系统，非模型生物的无细胞平台和用于快速评估生物学设计的多重策略。这些进展为实现合成基因网络的模型驱动设计，化合物的快速和便携式感测，按需生物制造，自下而上构建细胞以及下一代教育等新方法提供了令人兴奋的机会，从而可以深刻地改变合成生物学。套件。

更新日期：2019-11-28

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