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Relationship between the source size at the diffuser plane and the longitudinal spatial coherence function of the optical coherence microscopy system.
Journal of the Optical Society of America A ( IF 1.4 ) Pub Date : 2019-12-01 , DOI: 10.1364/josaa.36.000d41
Kashif Usmani , Azeem Ahmad , Rakesh Joshi , Vishesh Dubey , Ankit Butola , Dalip Singh Mehta

Coherence properties of light sources are indispensable for optical coherence microscopy/tomography as they greatly influence the signal-to-noise ratio, axial resolution, and penetration depth of the system. In the present paper, we report the investigation of longitudinal spatial coherence properties of a pseudothermal light source (PTS) as a function of the laser spot size at the rotating diffuser plate. The laser spot size is varied by translating a microscope objective lens toward or away from the diffuser plate. The longitudinal spatial coherence length, which governs the axial resolution of the coherence microscope, is found to be minimum for the beam spot size of 3.5 mm at the diffuser plate. The axial resolution of the system is found to be equal to an $\sim{13}\,\,{\rm \unicode{x00B5}{\rm m}}$∼13µm at 3.5 mm beam spot size. The change in the axial resolution of the system is confirmed by performing the experiments on standard gauge blocks of a height difference of 15 µm by varying the spot size at the diffuser plate. Thus, by appropriately choosing the beam spot size at the diffuser plane, any monochromatic laser light source can be utilized to obtain high axial resolution irrespective of the source's temporal coherence length. It can provide speckle-free tomographic images of multilayered biological specimens with large penetration depth. In addition, a PTS avoids the use of any chromatic-aberration-corrected optics and dispersion-compensation mechanism unlike conventional setups.

中文翻译:

漫射器平面上的光源尺寸与光学相干显微镜系统的纵向空间相干函数之间的关系。

光源的相干特性对于光学相干显微镜/断层扫描是必不可少的,因为它们会极大地影响系统的信噪比,轴向分辨率和穿透深度。在本文中,我们报告了对假热光源(PTS)的纵向空间相干特性的研究,该特性是旋转扩散板上激光光斑大小的函数。通过将显微镜物镜移向或远离扩散板来改变激光光斑的大小。对于在扩散板处3.5 mm的束斑尺寸,控制相干显微镜轴向分辨率的纵向空间相干长度最小。发现系统的轴向分辨率等于在3.5 mm束斑尺寸下的$ \ sim {13} \,\,{\ rm \ unicode {x00B5} {\ rm m}} $〜13µm。通过改变扩散板上的光点尺寸,在高度差为15 µm的标准量块上进行实验,可以确认系统轴向分辨率的变化。因此,通过适当地选择在扩散器平面上的束斑尺寸,可以使用任何单色激光光源来获得高轴向分辨率,而与光源的时间相干长度无关。它可以提供具有较大穿透深度的多层生物标本的无斑点断层图像。另外,与传统设置不同,PTS避免了使用任何色差校正光学器件和色散补偿机制。因此,通过适当地选择在扩散器平面上的束斑尺寸,可以使用任何单色激光光源来获得高轴向分辨率,而与光源的时间相干长度无关。它可以提供具有较大穿透深度的多层生物标本的无斑点断层图像。另外,与传统设置不同,PTS避免了使用任何色差校正光学器件和色散补偿机制。因此,通过适当地选择在扩散器平面上的束斑尺寸,可以使用任何单色激光光源来获得高轴向分辨率,而与光源的时间相干长度无关。它可以提供具有较大穿透深度的多层生物标本的无斑点断层图像。另外,与传统设置不同,PTS避免了使用任何色差校正光学器件和色散补偿机制。
更新日期:2019-11-28
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