A simple and direct atomic absorption spectrometry method for the direct determination of Hg in dried blood spots and dried urine spots prepared using various microsampling devices†,Journal of Analytical Atomic Spectrometry

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A simple and direct atomic absorption spectrometry method for the direct determination of Hg in dried blood spots and dried urine spots prepared using various microsampling devices†
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2019-11-27 , DOI: 10.1039/c9ja00348g
Flávio V. Nakadi _{1,

2,

3,

4} , Raúl Garde _{1,

2,

3,

4} , Márcia A. M. S. da Veiga _{5,

6,

7,

8,

9} , Julio Cruces _{4,

10,

11} , Martín Resano _{1,

2,

3,

4}

Affiliation

The use of dried matrix spots, such as dried blood spots (DBSs) and dried urine spots (DUSs), is becoming more popular in clinical analyses, beyond their traditional use for screening newborns. Currently, there are new types of microsampling devices that have been designed to collect a low and known volume of biological fluids, regardless of the hematocrit level, thus making it feasible to develop fully quantitative methods for their analysis. In this study, three of the most promising microsampling devices (Capitainer B, Hemaxis DB 10 and Mitra) were evaluated aiming at the direct determination of Hg using a dedicated “analyzer” based on atomic absorption spectrometry. Whole blood and urine reference materials were used to evaluate the methods developed. This Hg analyzer possesses a gold trap to enhance the sensitivity and minimize matrix effects, enabling direct analysis of the solid DBS and DUS samples using calibration with Hg aqueous solutions, with an instrumental limit of detection (LOD) and quantification (LOQ) of 3.4 and 11 pg, respectively. LODs when using the three microsampling devices were similar, ranging between 2.5 and 3.2 μg L⁻¹. It is also demonstrated that the simultaneous analysis of four Mitra derived DBSs in a quartz boat results in a decrease of the LOD to 0.32 μg L⁻¹.

中文翻译：

一种简单直接的原子吸收光谱法，用于直接测定使用各种微量采样设备制备的干血斑和干尿斑中的汞†

在临床分析中，使用干基质斑点（例如干血斑（DBS）和干尿斑点（DUSs））已变得越来越普遍，已超出了传统的筛查新生儿的用途。当前，已经设计了新型的微量采样装置，以收集少量已知的生物流体，而与血细胞比容水平无关，因此使开发完全定量的方法进行分析成为可能。在这项研究中，对三个最有前途的微量采样设备（Capitainer B，Hemaxis DB 10和Mitra）进行了评估，目的是使用基于原子吸收光谱法的专用“分析仪”直接测定汞。全血和尿液参考材料用于评估开发的方法。这款汞分析仪拥有一个金阱，可以提高灵敏度并最大程度地减少基体效应，通过使用汞水溶液进行校准，可以直接分析固体DBS和DUS样品，仪器的检测限（LOD）和定量限（LOQ）分别为3.4和11 pg。使用三个微量采样设备时的LOD相似，范围为2.5至3.2μgL^-1。还证明了在石英舟中对四个Mitra衍生的DBS的同时分析导致LOD降低至0.32μgL^-1。

更新日期：2020-01-02

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