To understand factors that influence the assembly of microbial communities, we inoculated Medicago sativa with a series of nested bacterial synthetic communities and grew plants in distinct nitrogen concentrations. Two isolates in our eight‐member synthetic community, Williamsia sp. R60 and Pantoea sp. R4, consistently dominate community structure across nitrogen regimes. While Pantoea sp. R4 consistently colonizes plants to a higher degree compared to the other six organisms across each community and each nutrient level, Williamsia sp. R60 exhibits nutrient specific colonization differences. Williamsia sp. R60 is more abundant in plants grown at higher nitrogen concentrations, but exhibits the opposite trend when no plant is present, indicating plant‐driven influence over colonization. Our research discovered unique, repeatable colonization phenotypes for individual microbes during plant microbiome assembly, and identified alterations caused by the host plant, microbes, and available nutrients.

中文翻译：

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区分苜蓿中营养依赖性植物驱动的细菌定殖模式。

为了了解影响微生物群落组装的因素，我们在苜蓿中接种了一系列嵌套的细菌合成群落，并在不同的氮浓度下种植了植物。我们八个成员的合成社区Williamsia sp。中的两个分离株。R60和Pantoea sp。R4始终主导着整个氮制度下的社区结构。虽然Pantoea sp。与每个群落和每个营养水平的其他六个生物（Williamsia sp。）相比，R4始终以较高的程度在植物上定植。R60表现出不同的营养定植差异。威廉西亚sp。R60在较高氮浓度下生长的植物中含量更高，但是当没有植物存在时却呈现相反的趋势，表明植物对定殖的影响。我们的研究发现了植物微生物组组装过程中单个微生物的独特，可重复的定殖表型，并确定了宿主植物，微生物和可用营养素引起的变化。