Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression.,Aging Cell

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Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression.
Aging Cell ( IF 8.0 ) Pub Date : 2019-11-26 , DOI: 10.1111/acel.13060
Yao Dang ₁ , Yongpan An ₁ , Jinzhao He ₁ , Boyue Huang ₁ , Jie Zhu ₁ , Miaomiao Gao ₁ , Shun Zhang ₁ , Xin Wang ₁ , Baoxue Yang _{1,

2} , Zhengwei Xie ₁

Affiliation

Although aging and senescence have been extensively studied in the past few decades, however, there is lack of clinical treatment available for anti‐aging. This study presents the effects of berberine (BBR) on the aging process resulting in a promising extension of lifespan in model organisms. BBR extended the replicative lifespan, improved the morphology, and boosted rejuvenation markers of replicative senescence in human fetal lung diploid fibroblasts (2BS and WI38). BBR also rescued senescent cells with late population doubling (PD). Furthermore, the senescence‐associated β‐galactosidase (SA‐β‐gal)‐positive cell rates of late PD cells grown in the BBR‐containing medium were ~72% lower than those of control cells, and its morphology resembled that of young cells. Mechanistically, BBR improved cell growth and proliferation by promoting entry of cell cycles from the G₀ or G₁ phase to S/G₂‐M phase. Most importantly, BBR extended the lifespan of chemotherapy‐treated mice and naturally aged mice by ~52% and ~16.49%, respectively. The residual lifespan of the naturally aged mice was extended by 80%, from 85.5 days to 154 days. The oral administration of BBR in mice resulted in significantly improved health span, fur density, and behavioral activity. Therefore, BBR may be an ideal candidate for the development of an anti‐aging medicine.

中文翻译：

小ber碱可通过调节p16和细胞周期蛋白的表达来改善细胞衰老并延长小鼠的寿命。

尽管在过去的几十年中已经对衰老和衰老进行了广泛的研究，但是，缺乏抗衰老的临床治疗方法。这项研究提出了小ber碱（BBR）对衰老过程的影响，导致模型生物的寿命有望延长。BBR延长了人类胎儿肺二倍体成纤维细胞（2BS和WI38）的复制寿命，改善了形态并增强了复制衰老的嫩化标记。BBR还通过后期种群倍增（PD）拯救了衰老细胞。此外，在含BBR的培养基中生长的晚期PD细胞的衰老相关β-半乳糖苷酶（SA-β-gal）阳性细胞率比对照细胞低约72％，其形态类似于年轻细胞。机械上，₀或G ₁相到S / G _2‐ M相。最重要的是，BBR分别将化疗治疗的小鼠和自然衰老的小鼠的寿命延长了〜52％和〜16.49％。自然衰老小鼠的剩余寿命从85.5天延长到154天，增加了80％。在小鼠中口服BBR可以显着改善健康期，毛皮密度和行为活动。因此，BBR可能是开发抗衰老药物的理想选择。

更新日期：2019-11-26

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