BACKGROUND Finding new biomarkers for the early detection of cervical cancer is an essential requirement in this field. In this study, we aimed to evaluate the expression level of potential biomarkers in progression of cervical cancer in patients with cervical cancer compared to normal subjects. METHODS The expression levels of tissue and serum miRNAs, including miR-9, miR-192 and miR-205, were investigated in 36 normal, 18 precancer, and 18 cervical cancer samples using real-time polymerase chain reaction. RESULTS The results showed the higher significant expressions of miR-9, miR-192 and miR-205 in the tissue of cancer samples than those in the normal samples. Moreover, the miR-192 and miR-205 expression were significantly increased in the cancer group in comparison with the precancer group. Examination of serum samples revealed the increase in the expression level in the cancer groups than in the normal samples, for miR-9, miR-192 and miR-205 and the expressions of miR-9, miR-192 and miR-205 were significantly up-regulated in the precancer group in comparison with the normal group. Also the expression of miR-205 was remarkably increased in the cancer group in comparison with the precancer group. The receiver operating characteristic (ROC) analyses showed the highest area under the curve value for miR-192. CONCLUSIONS Given the increased expression level of miR-192 in cancer and in precancerous tissue and serum compared with the normal tissue and serum validated by analysing the ROC curve, miR-192 can be used as potential biomarker for the early detection of cervical cancer.

中文翻译：

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血清和组织miRNA：诊断宫颈癌的潜在生物标志物。

背景技术在宫颈癌的早期发现中寻找新的生物标记物是该领域的基本要求。在这项研究中，我们旨在评估与正常受试者相比在宫颈癌患者中宫颈癌进展中潜在生物标志物的表达水平。方法使用实时聚合酶链反应，在36例正常，18例癌前期和18例宫颈癌样本中研究了组织和血清miRNA的表达水平，包括miR-9，miR-192和miR-205。结果结果表明，在癌组织中，miR-9，miR-192和miR-205的显着表达高于正常组织。此外，与癌前组相比，癌症组中的miR-192和miR-205表达显着增加。血清样品的检查显示，对于癌症组，miR-9，miR-192和miR-205的表达水平比正常样品有所增加，并且miR-9，miR-192和miR-205的表达显着增加与正常组相比，癌前组中的蛋白上调。与癌前组相比，在癌症组中miR-205的表达也显着增加。接收器工作特性（ROC）分析显示miR-192的曲线值下面积最大。结论通过分析ROC曲线证实，与正常组织和血清相比，miR-192在癌症以及癌前组织和血清中的表达水平有所提高，因此miR-192可作为宫颈癌早期检测的潜在生物标志物。