Yersinia ruckeri is the causative agent of enteric redmouth disease in salmonids. In fish, the intestine represents an important site of nutrient uptake, host-pathogen interactions, and defense. The posterior intestine can be inflamed, reddened, and filled with an opaque, yellowish fluid during Y. ruckeri infection. Herein, we report an investigation on the proteome alteration in the posterior intestinal mucosa of rainbow trout (Oncorhynchus mykiss) after exposure to Y. ruckeri. The intestinal mucosal proteins were identified and quantified by a shotgun proteomic approach by applying data-independent quantification with sequential windowed acquisition of all theoretical mass spectra (SWATH). A total of 437 proteins were found to be differentially up- or downregulated in the posterior intestine. Gene ontology of upregulated proteins pointed to their involvement into exopeptidase, endopeptidase, and hydrolase activities, while the downregulated proteins were involved in lipid metabolism, actin binding, and translation processes. Additionally, upregulated proteins were predicted to be involved in lysosome, oxidative phosphorylation, and metabolic pathways, while downregulated proteins were implicated in focal adhesion, regulation of actin cytoskeleton, protein digestion and absorption pathways. This study showed that Y. ruckeri infection can alter protein abundance involved in serine-type carboxypeptidase, cysteine and aspartic-type endopeptidases, metallopeptidases, antioxidant defense, calcium ion binding, glycolytic and carbohydrate metabolic processes in the proteome of the intestinal mucosa of rainbow trout.

中文翻译：

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鲁氏耶尔森氏菌感染后虹鳟鱼（Oncorhynchus mykiss）后肠粘膜蛋白质组的调节。

耶尔森氏菌是鲑鱼肠道红嘴病的病原体。在鱼类中，肠代表营养吸收，宿主-病原体相互作用和防御的重要部位。在鲁氏耶尔森氏菌感染期间，后肠会发炎，变红并充满不透明的淡黄色液体。在这里，我们报告调查暴露于ruckeri的虹鳟鱼（Oncorhynchus mykiss）后肠粘膜中的蛋白质组变化的调查。通过应用独立于数据的定量和顺序窗口采集所有理论质谱（SWATH），通过a弹枪蛋白质组学方法鉴定和定量肠粘膜蛋白。发现总共437种蛋白质在后肠中差异性上调或下调。上调蛋白的基因本体论指出它们参与外肽酶，内肽酶和水解酶活性，而下调的蛋白则参与脂质代谢，肌动蛋白结合和翻译过程。此外，预计上调的蛋白与溶酶体，氧化磷酸化和代谢途径有关，而下调的蛋白与粘着斑，肌动蛋白细胞骨架的调节，蛋白的消化和吸收途径有关。这项研究表明，ruckeri感染可以改变虹鳟鱼肠黏膜蛋白质组中丝氨酸型羧肽酶，半胱氨酸和天冬氨酸型内肽酶，金属肽酶，抗氧化防御，钙离子结合，糖酵解和碳水化合物代谢过程中涉及的蛋白质丰度。 。