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Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression
Retrovirology ( IF 2.7 ) Pub Date : 2018-09-21 , DOI: 10.1186/s12977-018-0447-9
Meng Wang , Wei Yang , Yu Chen , Jian Wang , Juan Tan , Wentao Qiao

BackgroundHuman immunodeficiency virus type 1 (HIV-1) Tat protein plays an essential role in HIV-1 gene transcription. Tat transactivates HIV-1 long terminal repeat (LTR)-directed gene expression through direct interactions with the transactivation-responsive region (TAR) element and other cis elements in the LTR. The TAR-independent Tat-mediated LTR transactivation is modulated by several host factors, but the mechanism is not fully understood.ResultsHere, we report that Tat interacts with the Rel homology domain of RelB through its core region. Furthermore, RelB significantly increases Tat-mediated transcription of the HIV-1 LTR and viral gene expression, which is independent of the TAR. Both Tat and RelB are recruited to the HIV-1 promoter, of which RelB facilitates the recruitment of Tat to the viral LTR. The NF-κB elements are key to the accumulation of Tat and RelB on the LTR. Knockout of RelB reduces the accumulation of RNA polymerase II on the LTR, and decreases HIV-1 gene transcription. Together, our data suggest that RelB contributes to HIV-1 transactivation.ConclusionsOur results demonstrate that RelB interacts with Tat and enhances TAR-independent activation of HIV-1 LTR promoter, which adds new insights into the multi-layered mechanisms of Tat in regulating the gene expression of HIV-1.

中文翻译:

细胞 RelB 与反式激活因子 Tat 相互作用并增强 HIV-1 表达

背景人类免疫缺陷病毒1型(HIV-1)Tat蛋白在HIV-1基因转录中起重要作用。Tat 通过与反式激活响应区 (TAR) 元件和 LTR 中的其他顺式元件直接相互作用,反式激活 HIV-1 长末端重复 (LTR) 导向的基因表达。不依赖于焦油的 Tat 介导的 LTR 反式激活受多种宿主因素调节,但机制尚未完全了解。结果在这里,我们报告 Tat 通过其核心区域与 RelB 的 Rel 同源域进行交互。此外,RelB 显着增加了 Tat 介导的 HIV-1 LTR 转录和病毒基因表达,这与 TAR 无关。Tat 和 RelB 都被招募到 HIV-1 启动子,其中 RelB 有助于将 Tat 招募到病毒 LTR。NF-κB 元件是 Tat 和 RelB 在 LTR 上积累的关键。敲除 RelB 会减少 LTR 上 RNA 聚合酶 II 的积累,并减少 HIV-1 基因转录。总之,我们的数据表明,RelB 有助于 HIV-1 反式激活。结论我们的结果表明,RelB 与 Tat 相互作用并增强了 HIV-1 LTR 启动子的 TAR 非依赖性激活,这为 Tat 在调节HIV-1的基因表达。
更新日期:2018-09-21
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