BackgroundWe previously showed that the gM of HSV-1 could restrict the release of infectious HIV-1 from cells. In this study, we analyzed if the four HSV-1 glycoproteins (gD, gB, and gH/gL), which are the minimum glycoproteins required for HSV-1 entry, restricted the release of infectious HIV-1.ResultsOf these four glycoproteins, gD and gH/gL restricted the production of infectious HIV-1 from cells transfected with an infectious molecular clone of HIV-1 (strain NL4-3) while gB had no significant effect. Pulse-chase analyses indicated that gD did not affect the biosynthesis and processing of gp160 into gp120/gp41, the transport of the gp120/gp41 to the cell surface, or the release of HIV-1 particles from the cell surface. Our analyses revealed that gD was incorporated into HIV-1 virus particles while gp120/gp41 was excluded from released virus particles. Truncated mutants of gD revealed that the cytoplasmic domain was dispensable but that a membrane bound gD was required for the restriction of release of infectious HIV-1. Finally, cell lines expressing gD also potently restricted the release of infectious virus.ConclusionsDue to its ability to exclude HIV-1 gp120/gp41 from maturing virus, gD may provide a useful tool in deciphering mechanisms of Env incorporation into maturing virus particles.

中文翻译：

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1 型单纯疱疹 gB、gD 和 gH/gL 对感染性 HIV-1 产生的分析：HSV-1 gD 通过从成熟病毒颗粒中排除 HIV-1 Env 来限制 HIV-1

背景我们之前表明 HSV-1 的 gM 可以限制感染性 HIV-1 从细胞中释放。在这项研究中，我们分析了四种 HSV-1 糖蛋白（gD、gB 和 gH/gL），它们是 HSV-1 进入所需的最少糖蛋白，是否限制了传染性 HIV-1 的释放。 结果这四种糖蛋白， gD 和 gH/gL 限制了感染性 HIV-1 从转染了 HIV-1（菌株 NL4-3）的细胞中产生，而 gB 没有显着影响。脉冲追踪分析表明，gD 不影响 gp160 的生物合成和加工成 gp120/gp41、gp120/gp41 向细胞表面的转运或 HIV-1 颗粒从细胞表面的释放。我们的分析表明，gD 被掺入到 HIV-1 病毒颗粒中，而 gp120/gp41 被排除在释放的病毒颗粒之外。gD 的截短突变体表明，细胞质域是可有可无的，但限制感染性 HIV-1 的释放需要结合膜的 gD。最后，表达 gD 的细胞系也有效地限制了传染性病毒的释放。结论 由于 gD 能够从成熟病毒中排除 HIV-1 gp120/gp41，gD 可能为破译 Env 掺入成熟病毒颗粒的机制提供有用的工具。