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Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss.
Reproductive Biology and Endocrinology ( IF 4.2 ) Pub Date : 2019-07-09 , DOI: 10.1186/s12958-019-0496-5
Dena Xue 1, 2 , Yi Zhang 2 , Yixin Wang 2 , Jun Wang 2 , Fengxiao An 2 , Xiaowei Sun 2 , Zhenhai Yu 2
Affiliation  

BACKGROUND Recurrent pregnancy loss (RPL) refers to two or more spontaneous abortions that occur consecutively with the same spouse. RPL severely affects human reproduction health, and causes extreme physical and mental suffering to patients and their families. METHODS We used isobaric tags for relative and absolute quantitation (iTRAQ), which was coupled with liquid chromatography mass spectrometry (LC-MS) proteomic analysis, in order to identify differentially expressed proteins. Moreover, we used western blot to analyze differentially expressed proteins. RESULTS Of the 2350 non-redundant proteins identified, 38 proteins were significantly altered and were identified as potential biomarkers for RPL. The protein-protein interaction network constructed using GeneMANIA revealed that 35.55% displayed similar co-expression, 30.87% were predicted, and 20.95% had physical interaction characteristics. Based on Gene ontology classification and KEGG pathway enrichment analyses, the majority of these differentially expressed proteins were found to be related to biological regulation, metabolic and cellular processes, protein binding and different enzymes activities, as well as disorder of fat and glucose metabolic pathways. It is noteworthy that three metabolism related biomarkers (HK1, ACLY, and FASN) were further confirmed through western blot analysis. CONCLUSIONS These results suggest that these differentially expressed proteins may be used as biomarkers for RPL, and related signaling pathways may play crucial roles in male induced RPL.

中文翻译:

无法解释的复发性流产中精子的定量蛋白质组学分析。

背景技术反复流产(RPL)是指由同一配偶连续发生的两次或更多次自然流产。RPL严重影响人类生殖健康,并给患者及其家人带来极大的身心痛苦。方法我们将等压标记用于相对和绝对定量(iTRAQ),并与液相色谱质谱(LC-MS)蛋白质组学结合,以鉴定差异表达的蛋白质。此外,我们使用蛋白质印迹法分析差异表达的蛋白质。结果在鉴定的2350种非冗余蛋白中,有38种蛋白发生了显着改变,并被确定为RPL的潜在生物标记。使用GeneMANIA构建的蛋白质-蛋白质相互作用网络显示35.55%表现出相似的共表达,预测为30.87%,有20.95%具有身体互动特征。根据基因本体分类和KEGG途径富集分析,发现这些差异表达的蛋白质中的大多数与生物调节,代谢和细胞过程,蛋白质结合和不同的酶活性以及脂肪和葡萄糖代谢途径的紊乱有关。值得注意的是,通过蛋白质印迹分析进一步证实了三种与代谢相关的生物标志物(HK1,ACLY和FASN)。结论这些结果表明,这些差异表达的蛋白质可以用作RPL的生物标志物,并且相关的信号通路可能在雄性诱导的RPL中起关键作用。发现这些差异表达的蛋白质中的大多数与生物调节,代谢和细胞过程,蛋白质结合和不同的酶活性以及脂肪和葡萄糖代谢途径的紊乱有关。值得注意的是,通过蛋白质印迹分析进一步证实了三种与代谢相关的生物标志物(HK1,ACLY和FASN)。结论这些结果表明,这些差异表达的蛋白质可以用作RPL的生物标志物,并且相关的信号通路可能在雄性诱导的RPL中起关键作用。发现这些差异表达的蛋白质中的大多数与生物调节,代谢和细胞过程,蛋白质结合和不同的酶活性以及脂肪和葡萄糖代谢途径的紊乱有关。值得注意的是,通过蛋白质印迹分析进一步证实了三种与代谢相关的生物标志物(HK1,ACLY和FASN)。结论这些结果表明,这些差异表达的蛋白质可以用作RPL的生物标志物,并且相关的信号通路可能在雄性诱导的RPL中起关键作用。通过蛋白质印迹分析进一步确认了ACLY和FASN)。结论这些结果表明,这些差异表达的蛋白质可以用作RPL的生物标志物,并且相关的信号通路可能在雄性诱导的RPL中起关键作用。通过蛋白质印迹分析进一步确认了ACLY和FASN)。结论这些结果表明,这些差异表达的蛋白质可以用作RPL的生物标志物,并且相关的信号通路可能在雄性诱导的RPL中起关键作用。
更新日期:2019-07-09
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