OBJECTIVE The objective ofthis study was to assess the association between thyroid hormone (TH) levels in follicular fluid (FF) and serum and to determine whether THs impact assisted reproductive technology (ART) outcomes. METHODS This study enrolled 299 women undergoing ART. Blood samples were drawn on the day of human chorionic gonadotrophin (HCG) administrationand analysed for thyroid-stimulating hormone (TSH), thyroxine(T4), triiodothyronine(T3),free T4 (fT4),free T3(fT3), thyroid peroxidase antibody (TPOAb) and thyroglobulin antibody (TGAb) levels. FF was obtained on the oocyte pick up (OPU) day and analysed forTSH, T4, T3, fT4, fT3, TPOAb, TgAb and estradiol levels. RESULTS (1) There were significant positive correlations between serum and FF TH and thyroid autoantibody levels. Statistically significant differences were discovered in serum and FF levels of TSH (p ≤ 0.001), T4 (p ≤ 0.001), T3 (p ≤ 0.001), TPOAbs (p ≤ 0.001) and TGAbs (p = 0.021). (2) Serum T4 levels [121.9(104.8,140.8) vs 114.1(98.6,130.6) nmol/l, p = 0.026], serum fT4 levels[(19.0(17.7,21.8) vs 18.6(17.0,20.1) pmol/l, p = 0.026], serum T4/T3 ratios [62.5 (55.7, 66.2) vs 59.4 (53.4, 64.9), p = 0.029], FF fT4 levels [19.0(17.5,21.3) vs 18.1(16.8,19.9) pmol/l, p = 0.009] and FF T4/T3 ratios [52.6 (46.4, 57.3) vs 50.0 (43.7, 53.1), p = 0.004] were significantly higher in the successful pregnancy group than the implantation failure group. (3) Spearman's rank correlation analysis revealed positive associations of both the FF T4/T3 ratio and serum TSH levels with the numbers of retrieved oocytes (total or MII) and embryos (fertilized, cleavage, and good quality). CONCLUSIONS TH levels in FF are strongly correlated with those in serum on the HCG day, and THs on the HCG day may affect ART outcomes.

中文翻译：

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血清和卵泡液甲状腺激素水平以及辅助生殖技术的结果。

目的本研究的目的是评估卵泡液（FF）和血清中甲状腺激素（TH）水平之间的关联，并确定THs是否影响辅助生殖技术（ART）的结果。方法该研究招募了299名接受ART治疗的女性。在服用人绒毛膜促性腺激素（HCG）当天抽取血样，并分析甲状腺刺激激素（TSH），甲状腺素（T4），三碘甲甲状腺素（T3），游离T4（fT4），游离T3（fT3），甲状腺过氧化物酶抗体（TPOAb）和甲状腺球蛋白抗体（TGAb）的水平。在卵母细胞摄取（OPU）日获得FF，并分析TSH，T4，T3，fT4，fT3，TPOAb，TgAb和雌二醇水平。结果（1）血清和FF TH与甲状腺自身抗体水平呈显着正相关。TSH（p≤0.001），T4（p≤0.001），T3（p≤0.001），TPOAbs（p≤0.001）和TGAbs（p = 0.021）的血清和FF水平存在统计学差异。（2）血清T4水平[121.9（104.8,140.8）vs 114.1（98.6,130.6）nmol / l，p = 0.026]，血清fT4水平[（19.0（17.7,21.8）vs 18.6（17.0,20.1）pmol / l ，p = 0.026]，血清T4 / T3比[62.5（55.7，66.2）vs 59.4（53.4，64.9），p = 0.029]，FF fT4水平[19.0（17.5,21.3）vs 18.1（16.8,19.9）pmol / l，p = 0.009]和FF T4 / T3比率[52.6（46.4，57.3）vs 50.0（43.7，53.1），p = 0.004]在妊娠成功组中显着高于植入失败组（3）Spearman's rank相关分析显示，FF T4 / T3比值和血清TSH水平与回收的卵母细胞（总数或MII）和胚胎（受精，卵裂，和良好的品质）。结论FF中的TH水平与HCG日血清水平密切相关，HCG日TH水平可能会影响ART的预后。