BACKGROUND Polycystic ovary syndrome is characterized by hyperactivity of the ovarian sympathetic nervous system, increases in the content and release of norepinephrine, as well as decreases in the number of β-adrenoreceptors. In the present study, β-adrenoreceptors in the ovaries of rats with polycystic ovary syndrome were blocked and analyzed the resultant effects on ovulation, hormone secretion and the enzymes responsible for the synthesis of catecholamines. METHODS At 60 days of age, vehicle or estradiol valerate-treated rats were injected with propranolol [10- 4 M] into the ovarian bursas on oestrus day. The animals were sacrificed on the next day of oestrus, and the ovulation response, the steroid hormone levels in the serum and the immunoreactivity of tyrosine hydroxylase and dopamine β-hydroxylase in the ovaries were measured. RESULTS In animals with the induction of polycystic ovary syndrome and β-adrenoreceptor blocking, ovulation was restored in more than half of the animals and resulted in decreased hyperandrogenism with respect to the levels observed in the estradiol valerate-treated group. Tyrosine hydroxylase and dopamine β-hydroxylase were present in the theca cells of the growing follicles and the interstitial gland. Injection of propranolol restored the tyrosine hydroxylase and ovarian dopamine β-hydroxylase levels in rats with polycystic ovary syndrome induction. CONCLUSIONS The results suggest that a single injection into the ovarian bursas of propranolol, a nonselective antagonist of β-adrenoreceptor receptors, decreases the serum testosterone concentration and the formation of ovarian cysts, improving the ovulation rate that accompanies lower levels of tyrosine hydroxylase and dopamine β-hydroxylase in the ovary.

中文翻译：

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在戊酸雌二醇诱发的多囊卵巢综合征大鼠中，卵巢β-肾上腺素受体的急性阻断可改善排卵。

背景技术多囊卵巢综合征的特征在于卵巢交感神经系统活动亢进，去甲肾上腺素的含量和释放增加以及β-肾上腺素受体的数量减少。在本研究中，对多囊卵巢综合征大鼠卵巢中的β-肾上腺素受体进行了阻断，并分析了其对排卵，激素分泌和负责儿茶酚胺合成的酶的影响。方法60日龄时，在媒介或戊酸雌二醇处理的大鼠在发情日将普萘洛尔[10-4 M]注射到卵巢囊中。在发情的第二天处死动物，并测量排卵反应，血清中类固醇激素水平以及卵巢中酪氨酸羟化酶和多巴胺β-羟化酶的免疫反应性。结果在诱发多囊卵巢综合征和β-肾上腺素受体阻滞的动物中，一半以上的动物恢复了排卵，并且相对于戊酸雌二醇治疗组的水平，导致雄激素过多症的发生率降低。酪氨酸羟化酶和多巴胺β-羟化酶存在于正在生长的卵泡和间质腺的卵泡膜细胞中。普萘洛尔注射可恢复多囊卵巢综合征诱发大鼠的酪氨酸羟化酶和卵巢多巴胺β-羟化酶水平。结论：结果表明，将普萘洛尔（一种非选择性的β-肾上腺素受体拮抗剂）注射入卵巢囊中，可降低血清睾丸激素浓度和卵巢囊肿的形成，