BACKGROUND Torque teno virus-induced aseptic meningitis has not been documented, although torque teno virus infections still remain under consideration for etiological agents. This study identified a torque teno virus sequence using next generation sequencing and immunoglobulin M response to the torque teno virus antigen, therefore, that would be a comprehensive diagnosis for torque teno virus infection. CASE PRESENTATION A 2-month-old Japanese boy was brought to our hospital because he was irritable, drowsy, and lethargic. He was admitted based on his test results which indicated the possibility of septic meningitis. He was started on treatment with high-dose antibiotics and steroids. On the third day of hospitalization, he became afebrile with improvement in his general status and was discharged on the sixth day. He had no developmental problems for up to 1 year after discharge. Metagenomic ribonucleic acid-Seq pathogen detection using next generation sequencing of a sample of his cerebrospinal fluid, which was collected at admission, revealed three short reads homologous to those in torque teno virus out of a total of 1,708,516 reads. This finding indicated that our patient was positive compared to the torque teno virus-negative cerebrospinal fluid samples (controls) from 13 other patients. The torque teno virus has been shown to have a whole genome sequence of 2810 nt by polymerase chain reaction. We prepared a recombinant GP2 antigen from torque teno virus and used it to study our patient's anti-torque teno virus immune response. An anti-GP2 serum immunoglobulin M response was detected, providing further supportive evidence of torque teno virus infection. CONCLUSIONS This case speculates that torque teno virus-induced aseptic meningitis has a good course. New technologies like next generation sequencing can help in the identification of such cases, and an accumulation of future cases is expected.

中文翻译：

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婴儿扭力腱病毒引起的无菌性脑膜炎：一例报告。

背景技术虽然转矩扭矩病毒感染仍然是病原体的考虑因素，但尚未报道转矩扭矩病毒引起的无菌性脑膜炎。这项研究使用下一代测序方法和扭矩球蛋白病毒抗原对免疫球蛋白M的反应鉴定了扭矩球蛋白病毒序列，因此，这将是对扭矩球蛋白病毒感染的综合诊断。病例介绍一个2个月大的日本男孩因烦躁，昏昏欲睡和嗜睡而被送往我们医院。根据他的测试结果被录取，这表明可能存在败血性脑膜炎。他开始接受大剂量抗生素和类固醇的治疗。在住院的第三天，他的病情随着总体状况的改善而改善，并在第六天出院。他出院后长达一年没有任何发育问题。使用入院时收集的他的脑脊液样品的下一代测序对超基因组核糖核酸-Seq病原体进行检测，结果显示在总共1,708,516个读数中，有三个与扭矩Teno病毒同源的短读。这一发现表明，与来自其他13位患者的扭矩Teno病毒阴性脑脊髓液样本（对照）相比，我们的患者呈阳性。通过聚合酶链反应显示，扭力腱病毒具有2810 nt的整个基因组序列。我们从扭力腱糖蛋白病毒制备了重组GP2抗原，并将其用于研究患者的反扭力腱糖蛋白病毒免疫反应。检测到抗GP2血清免疫球蛋白M反应，为扭矩腱蛋白病毒感染提供了进一步的支持证据。结论该病例推测扭力腱糖蛋白病毒引起的无菌性脑膜炎具有良好的病程。下一代测序之类的新技术可以帮助识别此类病例，并且预计将来还会出现大量病例。