Immunosenescence, i.e. the aging-associated decline of the capacity of the immune system, is characterized by several distinct changes in the number and functions of the immune cells. In the case of B cells, the total number of CD19+ B cells is lower in the blood of elderly individuals than in the younger ones. CD19+ B cell population contains several subsets, which are commonly characterized by the presence of CD27 and IgD molecules, i.e. naïve B cells (CD27- IgD+), IgM memory (CD27+ IgD+), switched memory (CD27+ IgD-) and late memory (CD27- IgD-). This late memory, double negative, population represents cells which are nondividing, but are still able to produce inflammatory mediators and in this way maybe contributing to the aging-associated inflammation, inflammaging. Here we have focused on the role of these B cell subsets in elderly individuals, nonagenarians, in the regulation of inflammation and inflammation-associated decline of bodily functions. As the biological aging process demonstrates gender-specific characteristics, the analyses were performed separately in males and female. A subcohort of The Vitality 90+ study (67 nonagenarians, 22/45 males/females and 40 young controls, 13/27 males/females) was used. Flow cytometric analysis indicated that the total percentage of the CD19+ cells was ca. 50% lower in the nonagenarians than in the controls in both genders. The proportions of these four B cell subsets within the CD19+ populations were very similar in young and old individuals. Thus, it seems that the aging-associated decline of the total CD19+ cells affects similarly all these B cell subsets. To analyze the role of these subsets in the regulation of inflammation, the correlation with IL-6 levels was calculated. A significant correlation was observed only with the percentage of CD27- IgD- cells and only in males. As inflammation is associated with aging-associated functional performance and frailty, the correlations with the Barthel index and frailty score was analyzed. Again, only the CD27- IgD- population demonstrated a strong male-specific correlation. These data show that the CD27- IgD- B cell subset demonstrates a strong pro-inflammatory effect in nonagenarians, which significantly associates with the decline of the bodily functions. However, this phenomenon is only observed in males.

中文翻译：

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CD27- IgD- B 细胞记忆亚群与老年人的炎症和虚弱有关，但仅在男性中

免疫衰老，即与衰老相关的免疫系统能力下降，其特征在于免疫细胞数量和功能的几个明显变化。就 B 细胞而言，老年人血液中 CD19+ B 细胞的总数低于年轻人。CD19+ B 细胞群包含几个亚群，通常以存在 CD27 和 IgD 分子为特征，即幼稚 B 细胞 (CD27-IgD+)、IgM 记忆 (CD27+ IgD+)、转换记忆 (CD27+ IgD-) 和晚期记忆 (CD27 - IgD-)。这种晚期记忆，双阴性，群体代表不分裂的细胞，但仍然能够产生炎症介质，并且以这种方式可能导致与衰老相关的炎症，炎症。在这里，我们专注于这些 B 细胞亚群在老年人、九岁老人中在调节炎症和炎症相关的身体功能下降中的作用。由于生物老化过程表现出性别特异性特征，因此对男性和女性分别进行了分析。使用了 Vitality 90+ 研究的一个亚组（67 名非老年患者，22/45 男性/女性和 40 名年轻对照，13/27 男性/女性）。流式细胞仪分析表明，CD19+ 细胞的总百分比约为。在两性中，非老年人比对照组低 50%。CD19+ 群体中这四个 B 细胞亚群的比例在年轻人和老年人中非常相似。因此，似乎与衰老相关的总 CD19+ 细胞下降同样影响了所有这些 B 细胞亚群。为了分析这些亚群在炎症调节中的作用，计算了与 IL-6 水平的相关性。仅与 CD27- IgD- 细胞的百分比且仅在男性中观察到显着相关性。由于炎症与衰老相关的功能表现和虚弱有关，因此分析了与 Barthel 指数和虚弱评分的相关性。同样，只有 CD27-IgD-群体表现出强烈的男性特异性相关性。这些数据表明，CD27-IgD-B 细胞亚群在九龄人中表现出强烈的促炎作用，这与身体功能的下降显着相关。然而，这种现象只在男性中观察到。仅与 CD27- IgD- 细胞的百分比且仅在男性中观察到显着相关性。由于炎症与衰老相关的功能表现和虚弱有关，因此分析了与 Barthel 指数和虚弱评分的相关性。同样，只有 CD27-IgD-群体表现出强烈的男性特异性相关性。这些数据表明，CD27-IgD-B 细胞亚群在九龄人中表现出强烈的促炎作用，这与身体功能的下降显着相关。然而，这种现象只在男性中观察到。仅与 CD27- IgD- 细胞的百分比且仅在男性中观察到显着相关性。由于炎症与衰老相关的功能表现和虚弱有关，因此分析了与 Barthel 指数和虚弱评分的相关性。同样，只有 CD27-IgD-群体表现出强烈的男性特异性相关性。这些数据表明，CD27-IgD-B 细胞亚群在九龄人中表现出强烈的促炎作用，这与身体功能的下降显着相关。然而，这种现象只在男性中观察到。只有 CD27- IgD- 群体表现出强烈的男性特异性相关性。这些数据表明，CD27-IgD-B 细胞亚群在九龄人中表现出强烈的促炎作用，这与身体功能的下降显着相关。然而，这种现象只在男性中观察到。只有 CD27- IgD- 群体表现出强烈的男性特异性相关性。这些数据表明，CD27-IgD-B 细胞亚群在九龄人中表现出强烈的促炎作用，这与身体功能的下降显着相关。然而，这种现象只在男性中观察到。