BackgroundChronic neuropathic pain is often associated with anxiety, depressive symptoms, and cognitive impairment with relevant impact on patients` health related quality of life. To investigate the influence of a pro-inflammatory phenotype on affective and cognitive behavior under neuropathic pain conditions, we assessed mice deficient of the B7 homolog 1 (B7-H1), a major inhibitor of inflammatory response.ResultsAdult B7-H1 ko mice and wildtype littermates (WT) received a chronic constriction injury (CCI) of the sciatic nerve, and we assessed mechanical and thermal sensitivity at selected time points. Both genotypes developed mechanical (p < 0.001) and heat hypersensitivity (p < 0.01) 7, 14, and 20 days after surgery. We performed three tests for anxiety-like behavior: the light–dark box, the elevated plus maze, and the open field. As supported by the results of these tests for anxiety-like behavior, no relevant differences were found between genotypes after CCI. Depression-like behavior was assessed using the forced swim test. Also, CCI had no effect on depression like behavior. For cognitive behavior, we applied the Morris water maze for spatial learning and memory and the novel object recognition test for object recognition, long-, and short-term memory. Learning and memory did not differ in B7-H1 ko and WT mice after CCI.ConclusionsOur study reveals that the impact of B7-H1 on affective-, depression-like- and learning-behavior, and memory performance might play a subordinate role in mice after nerve lesion.

中文翻译：

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B7-H1缺陷型和野生型小鼠的慢性收缩损伤不会改变情感和认知行为

背景慢性神经性疼痛通常与焦虑、抑郁症状和认知障碍相关，对患者的健康相关生活质量有相关影响。为了研究在神经性疼痛条件下促炎表型对情感和认知行为的影响，我们评估了缺乏 B7 同源物 1 (B7-H1) 的小鼠，这是一种炎症反应的主要抑制剂。结果成年 B7-H1 ko 小鼠和野生型同窝仔 (WT) 的坐骨神经受到慢性压迫性损伤 (CCI)，我们在选定的时间点评估了机械和热敏感性。两种基因型都在手术后 7、14 和 20 天出现机械性 (p < 0.001) 和热超敏性 (p < 0.01)。我们对类似焦虑的行为进行了三项测试：明暗箱、高架十字迷宫和空旷场地。正如这些焦虑样行为测试的结果所支持的那样，CCI 后基因型之间没有发现相关差异。使用强迫游泳测试评估抑郁样行为。此外，CCI 对抑郁样行为没有影响。对于认知行为，我们将莫里斯水迷宫应用于空间学习和记忆，并将新的物体识别测试应用于物体识别、长期和短期记忆。CCI 后 B7-H1 ko 和 WT 小鼠的学习和记忆没有差异。结论我们的研究表明，B7-H1 对情感、抑郁样和学习行为以及记忆表现的影响可能在小鼠中起次要作用神经损伤后。使用强迫游泳测试评估抑郁样行为。此外，CCI 对抑郁样行为没有影响。对于认知行为，我们将莫里斯水迷宫应用于空间学习和记忆，并将新的物体识别测试应用于物体识别、长期和短期记忆。CCI 后 B7-H1 ko 和 WT 小鼠的学习和记忆没有差异。结论我们的研究表明，B7-H1 对情感、抑郁样和学习行为以及记忆表现的影响可能在小鼠中起次要作用神经损伤后。使用强迫游泳测试评估抑郁样行为。此外，CCI 对抑郁样行为没有影响。对于认知行为，我们将莫里斯水迷宫应用于空间学习和记忆，并将新的物体识别测试应用于物体识别、长期和短期记忆。CCI 后 B7-H1 ko 和 WT 小鼠的学习和记忆没有差异。结论我们的研究表明，B7-H1 对情感、抑郁样和学习行为以及记忆表现的影响可能在小鼠中起次要作用神经损伤后。