BackgroundDespite increasing evidence that lipopolysaccharide (LPS) affects the biological active substances of dorsal root ganglia (DRG) we have limited knowledge of the influence of a single low dose of LPS, which does not result in any clinical symptoms of disease (subclinical LPS) on neuropeptides connected with the sensory pathway. Accordingly, in this work, we investigated the influence of subclinical LPS from Salmonella Enteritidis on selected neuropeptides: substance P (SP), galanin (GAL), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and somatostatin (SOM) in the cervical, thoracic, lumbar and sacral regions of the DRG and spinal cord.MethodsThis study was performed on immature female pigs of the Pietrain × Duroc breed. Seven days after the intravenous injection of saline solution for control animals (n = 5) and 5 μg/kg b.w. LPS from S. Enteritidis for the experimental group (n = 5), the DRG and the spinal cord were collected to extract the neuropeptides using solid-phase extraction technology.ResultsOur results demonstrated that subclinical LPS in DRG was able to change the levels of all studied neuropeptides except SOM, whereas in the spinal cord it down-regulated all studied neuropeptides in the sacral spinal cord, maintaining the concentration of all studied neuropeptides in other regions similar to that observed in the control animals. The significant differences in the intensity and character of observed changes between particular regions of the DRG suggest that the exact functions of the studied neuropeptides and mechanisms of responses to subclinical LPS action depend on specific characteristics and functions of each examination region of DRG.ConclusionsThe mechanisms of observed changes are not fully understood and require further study of the molecular interactions between subclinical LPS from S. Enteritidis and neuronal and non-neuronal cells of DRG and spinal cord. The peripheral and central pain pathways must be analysed with the aspect of unknown long-term consequences of the influence of subclinical LPS from S. Enteritidis on neuropeptides in the spinal cord and the dorsal root ganglia.

中文翻译：

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肠炎沙门氏菌亚临床脂多糖诱导脊髓和背根神经节神经肽失调

背景尽管越来越多的证据表明脂多糖 (LPS) 影响背根神经节 (DRG) 的生物活性物质，但我们对单次低剂量 LPS 的影响知之甚少，这不会导致任何疾病的临床症状（亚临床 LPS）与感觉通路相连的神经肽。因此，在这项工作中，我们研究了肠炎沙门氏菌的亚临床 LPS 对选定神经肽的影响：物质 P (SP)、甘丙肽 (GAL)、神经肽 Y (NPY)、血管活性肠肽 (VIP) 和生长抑素 (SOM)。 DRG 和脊髓的颈、胸、腰和骶区域。方法本研究在 Pietrain × Duroc 品种的未成熟雌性猪上进行。对照动物 (n = 5) 和 5 μg/kg bw 静脉注射生理盐水后 7 天 实验组肠炎沙门氏菌LPS（n = 5），收集DRG和脊髓以固相提取技术提取神经肽。 结果我们的结果表明DRG中的亚临床LPS能够改变所有研究了除 SOM 之外的神经肽，而在脊髓中，它下调了骶脊髓中所有研究的神经肽，使所有研究的神经肽在其他区域的浓度保持与在对照动物中观察到的相似。DRG 特定区域之间观察到的变化的强度和特征的显着差异表明所研究的神经肽的确切功能和对亚临床 LPS 作用的反应机制取决于 DRG 的每个检查区域的特定特征和功能。结论 观察到的变化的机制尚不完全清楚，需要进一步研究肠炎沙门氏菌的亚临床 LPS 与 DRG 和脊髓的神经元和非神经元细胞之间的分子相互作用。必须从肠炎沙门氏菌的亚临床 LPS 对脊髓和背根神经节中的神经肽的影响的未知长期后果方面来分析外周和中枢疼痛通路。