The manual classification of protein domains is approaching its 20th anniversary. ECOD is our mixed manual-automatic domain classification. Over time, the types of proteins which require manual curation has changed. Depositions with complex multidomain and multichain arrangements are commonplace. Transmembrane domains are regularly classified. Repeatedly, domains which are initially believed to be novel are found to have homologous links to existing classified domains. Here we present a brief summary of recent manual curation efforts in ECOD generally combined with specific case studies of transmembrane and multidomain proteins wherein manual curation was useful for discovering new homologous relationships. We present a new taxonomy for the classification of ABC transporter transmembrane domains. We examine alternate topologies of the leucine-specific (LS) domain of Leucine tRNA-synthetase. Finally, we elaborate on a distant homologous links between two helical dimerization domains.

中文翻译：

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ECOD：多域和跨膜域蛋白之间远距离同源性的鉴定


蛋白质结构域的手动分类即将迎来 20 周年。 ECOD 是我们的混合手动-自动域分类。随着时间的推移，需要手动管理的蛋白质类型发生了变化。复杂的多域和多链排列的沉积是很常见的。跨膜结构域被定期分类。反复地，最初被认为是新颖的域被发现与现有的分类域具有同源链接。在这里，我们简要总结了最近在 ECOD 中的手动管理工作，通常与跨膜和多域蛋白的具体案例研究相结合，其中手动管理对于发现新的同源关系很有用。我们提出了一种新的 ABC 转运蛋白跨膜结构域分类法。我们研究了亮氨酸 tRNA 合成酶的亮氨酸特异性 (LS) 结构域的替代拓扑。最后，我们详细阐述了两个螺旋二聚化结构域之间的远距离同源联系。