BACKGROUND Microtubule proteins are able to produce electromagnetic fields and have an important role in memory formation, and learning. Therefore, microtubules have the potential to be affected by exogenous electromagnetic fields. This study aimed to examine the comparison of microtubule polymerization and its structural behavior in brain and sperm affected by 50 Hz extremely low-frequency electromagnetic field (ELEF). RESULTS Twenties adult male rats were randomly and equally divided into control and experimental groups, to evaluate the effect of 50 Hz ELEF on the sperm and brain functions. Plus-maze, serum testosterone and corticosterone, and sperm evaluation were performed. Next, the semen and brain samples were obtained, and they were divided into four experimental groups for investigation of microtubule polymerization. There was no significant difference in testosterone and, corticosterone levels, anxiety behaviors, and sperm morphology between control and ELEF-exposure groups. The sperm viability, total and progressive motility were significantly higher in the ELEF-exposed group than that of the control group. The microtubule polymerization in sperm ELEF was significantly higher than in other groups. The secondary and tertiary structures of tubulins were significantly affected in the brain, and sperm ELEF groups. CONCLUSION It seems that the polymerization of microtubules and conformational changes of tubulin dimers are improved by ELEF application.

中文翻译：

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比较极低频电磁场对大鼠脑和精子中微管聚合和结构行为的影响。

背景技术微管蛋白能够产生电磁场，并且在记忆形成和学习中具有重要作用。因此，微管有可能受到外源电磁场的影响。这项研究旨在检查微管聚合及其在受50 Hz超低频电磁场（ELEF）影响的脑和精子中的结构行为的比较。结果将20只成年雄性大鼠随机分为实验组和对照组，以评估50 Hz ELEF对精子和脑功能的影响。进行迷宫，血清睾丸激素和皮质酮以及精子评估。接下来，获得精液和脑样本，并将它们分为四个实验组以研究微管聚合。在对照组和ELEF暴露组之间，睾丸激素和皮质酮水平，焦虑行为和精子形态没有显着差异。ELEF暴露组的精子活力，总运动和进行性运动明显高于对照组。精子ELEF中的微管聚合显着高于其他组。微管蛋白的二级和三级结构在大脑和精子ELEF组中均受到显着影响。结论似乎通过ELEF的应用可以改善微管的聚合和微管蛋白二聚体的构象变化。ELEF暴露组的总运动能力和进行性运动明显高于对照组。精子ELEF中的微管聚合显着高于其他组。微管蛋白的二级和三级结构在大脑和精子ELEF组中均受到显着影响。结论似乎通过ELEF的应用可以改善微管的聚合和微管蛋白二聚体的构象变化。ELEF暴露组的总运动能力和进行性运动明显高于对照组。精子ELEF中的微管聚合显着高于其他组。微管蛋白的二级和三级结构在大脑和精子ELEF组中均受到显着影响。结论似乎通过ELEF的应用可以改善微管的聚合和微管蛋白二聚体的构象变化。