Matrix mineralization is a key stage in bone formation involving in many bone-specific genes and signaling pathways. Emerging evidence indicate that long non-coding RNA (lncRNA) and microRNAs (miRNAs) play crucial roles in regulating the mineralization process of osteoblasts. This study aims to characterize the function and mechanism of lncRNA H19/miR-185-5p/IGF1 axis in modulating matrix mineralization of osteoblasts. H19 and IGF1 were highly expressed while miR-185-5p was lowly expressed in mineralized cells. Knocking down H19 inhibited matrix mineralization of osteoblasts, yet miR-185-5p had opposite effects. Moreover, H19 directly targeted miR-185-5p, whereas miR-185-5p repressed IGF1 expression. Meanwhile, miR-185-5p inhibition compensated the suppression of the matrix mineralization in osteoblasts by H19 knockdown. The findings of this study showed that lncRNA H19 was upregulated in mineralized osteoblasts and promoted matrix mineralization through miR-185-5p/IGF1 axis in osteoblasts for the first time. This study may provide a new perspective for the diagnosis and treatment of diseases related to bone metabolism.

中文翻译：

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lncRNA H19通过上调成骨细胞中的miR-185-5p来上调IGF1来促进基质矿化

基质矿化是骨骼形成的关键阶段，涉及许多骨骼特异性基因和信号通路。越来越多的证据表明，长的非编码RNA（lncRNA）和microRNA（miRNA）在调节成骨细胞的矿化过程中起着至关重要的作用。本研究旨在表征lncRNA H19 / miR-185-5p / IGF1轴在调节成骨细胞基质矿化中的功能和机制。在矿化细胞中，H19和IGF1高表达，而miR-185-5p低表达。敲低H19可抑制成骨细胞基质矿化，而miR-185-5p具有相反的作用。此外，H19直接靶向miR-185-5p，而miR-185-5p抑制了IGF1表达。同时，miR-185-5p的抑制作用补偿了H19敲低对成骨细胞基质矿化的抑制作用。这项研究的发现表明，lncRNA H19在成矿成骨细胞中被上调，并通过成骨细胞中的miR-185-5p / IGF1轴促进了基质矿化。该研究可能为骨代谢相关疾病的诊断和治疗提供新的视角。