Mannose-binding lectin (MBL) plays an important role in the innate immune response by activating the complement system via the lectin pathway, and it has been studied in several viral infections; however, the influence of MBL in PLWHA infected with HHV-8 is unknown. The objective of this study was to verify the association of MBL deficient plasma concentrations in HIV/HHV-8 coinfected and HIV monoinfected patients and to correlate these concentrations with HIV viral load and CD4 counts in both groups. This was an analytical study of case-controls consisting of PLWHA monitored at the medical outpatient of Infectious and Parasitic Diseases of the clinical hospital in the Federal University of Pernambuco. Plasma concentrations of MBL were obtained by an enzyme-linked immunosorbent assay (ELISA) using a commercial Human Mannose Binding Lectin kit (MyBioSource, Inc.) that was performed according to the manufacturer’s guidelines, with values < 100 ng/ml considered deficient. A total of 245 PLWHA samples were analysed; 118 were HIV/HHV-8 coinfected and 127 were HIV monoinfected; 5.1% (6/118) of the coinfected patients and 3.2% (4/127) of the monoinfected patients (p = 0.445) were considered plasma concentration deficient. The median of the plasma concentrations of MBL in the coinfected patients was 2803 log10 ng/ml and was 2.959 log10 ng/ml in the monoinfected patients (p = 0.001). There was an inverse correlation between the plasma concentrations of MBL and the HIV viral load in both groups, but no correlation with the CD4 count. Although the plasma concentrations considered deficient in MBL were not associated with HHV-8 infection in PLWHA, the coinfected patients showed lower MBL concentrations and an inverse correlation with HIV viral load, suggesting that there may be consumption and reduction of MBL due to opsonization of HIV and HHV-8, leading to the reduction of plasma MBL and non-accumulation in the circulation.

中文翻译：

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感染HHV-8的艾滋病毒/艾滋病患者中与甘露糖结合的凝集素浓度

甘露糖结合凝集素（MBL）通过凝集素途径激活补体系统，在先天性免疫应答中起重要作用，并且已经在多种病毒感染中进行了研究。但是，MBL对感染了HHV-8的PLWHA的影响尚不清楚。这项研究的目的是验证合并感染的HIV / HHV-8和感染HIV的患者中MBL缺乏血浆浓度的相关性，并将这些浓度与两组中的HIV病毒载量和CD4计数相关联。这是对由伯南布哥联邦大学临床医院传染病和寄生虫病门诊患者监测的由PLWHA组成的病例对照研究的分析研究。MBL的血浆浓度是通过酶联免疫吸附测定（ELISA）使用商购的Mannose Binding Lectin试剂盒（MyBioSource，Inc.）根据制造商的指导原则进行测定的，其值<100 ng / ml被认为是不足的。总共分析了245个PLWHA样品；HIV / HHV-8共感染118例，HIV单一感染127例；合并感染的患者中有5.1％（6/118），单感染的患者中有3.2％（4/127）（p = 0.445）被认为血浆浓度不足。合并感染患者的MBL血浆中位数为2803 log10 ng / ml，单感染患者为2.959 log10 ng / ml（p = 0.001）。两组的MBL血浆浓度和HIV病毒载量之间呈负相关，但与CD4计数无相关性。