BACKGROUND Multiple sclerosis is a demyelinating and autoimmune disease and its immune response is not fully elucidated. This study was conducted to examine the pathological changes and B cell subsets in experimental autoimmune encephalomyelitis (EAE) mice, and analyze the expression of triosephosphate isomerase (TPI) and GADPH to define the role of B cell subsets in the disease. RESULTS Female C57BL/6 mice were randomly divided into EAE group (n = 18) and control (n = 18). During the experiments, the weight and nerve function scores were determined. The proportions of B cell subsets in the peripheral blood were measured by flow cytometry. Seven, 18 and 30 days after immunization, the brain and spinal cord tissues were examined for the infiltration of inflammatory cells using hematoxylin-eosin (HE) HE staining and the demyelination using Luxol fast blue staining. The expression of B cell-related proteins was detected immunohistochemistrially and the expression of antigenic TPI and GADPH was analyzed using enzyme-linked immunosorbent assay (ELISA). HE staining showed that mice had more severe EAE 18 d than 7 d after modelling, while the symptoms were significantly relieved at 30 d. The results were consistent with the weight measurements and neural function scores. Immunohistochemistry studies showed that B cells aggregated in the spinal cord, but not much in the brain. Flow cytometry studies showed that there were more B cells in control than in EAE models from day 7 and the difference was narrowed at day 30. The level of plasma cells increased continuously, reached the top at day 21 and obviously declined at day 30. On other hand, the numbers of memory B cells increased gradually over the experimental period. The numbers of plasma and memory B cells were similar between the control and EAE mice. ELISA data revealed that the brain contents of TPI and GAPDH were higher in EAE mice than in control at day 7, while at day 18, the levels were reversed. CONCLUSIONS In the central pathological process of EAE mice, B cells exert role through the mechanism other than producing antibodies and the levels of brain TPI and GADPH are related to the severity of autoimmune induced-damage.

中文翻译：

---

小鼠自身免疫性脑脊髓炎的中央病理过程中B细胞亚群的变化。

背景技术多发性硬化症是脱髓鞘性和自身免疫性疾病，并且其免疫应答尚未完全阐明。这项研究旨在检查实验性自身免疫性脑脊髓炎（EAE）小鼠的病理变化和B细胞亚群，并分析磷酸三糖异构酶（TPI）和GADPH的表达，以定义B细胞亚群在该疾病中的作用。结果雌性C57BL / 6小鼠被随机分为EAE组（n = 18）和对照组（n = 18）。在实验过程中，确定了体重和神经功能评分。通过流式细胞术测量外周血中B细胞亚群的比例。免疫后7天，18天和30天，使用苏木精-曙红（HE）HE染色检查脑和脊髓组织中炎性细胞的浸润，并使用Luxol固蓝染色检查脱髓鞘。免疫组化检测B细胞相关蛋白的表达，并用酶联免疫吸附试验（ELISA）分析抗原性TPI和GADPH的表达。HE染色表明，小鼠在建模后18 d比7 d具有更严重的EAE，而在30 d时症状显着缓解。结果与体重测量和神经功能评分一致。免疫组织化学研究表明，B细胞聚集在脊髓中，但在大脑中聚集较少。流式细胞仪研究表明，从第7天开始，对照中的B细胞比EAE模型中的B细胞更多，并且在第30天时差异有所缩小。浆细胞的水平持续增加，在第21天达到顶部，并在第30天明显下降。另一方面，记忆B细胞的数量在实验期间逐渐增加。在对照和EAE小鼠之间，血浆和记忆B细胞的数目相似。ELISA数据显示，在第7天，EAE小鼠的TPI和GAPDH的大脑含量高于对照组，而在第18天，其水平却相反。结论在EAE小鼠的中央病理过程中，B细胞通过除产生抗体以外的机制发挥作用，并且脑TPI和GADPH的水平与自身免疫诱导损伤的严重程度有关。在整个实验过程中，记忆B细胞的数量逐渐增加。在对照和EAE小鼠之间，血浆和记忆B细胞的数目相似。ELISA数据显示，在第7天，EAE小鼠的TPI和GAPDH的脑内含量高于对照组，而在第18天，其水平则相反。结论在EAE小鼠的中央病理过程中，B细胞通过除产生抗体以外的机制发挥作用，并且脑TPI和GADPH的水平与自身免疫诱导损伤的严重程度有关。在整个实验过程中，记忆B细胞的数量逐渐增加。在对照和EAE小鼠之间，血浆和记忆B细胞的数目相似。ELISA数据显示，在第7天，EAE小鼠的TPI和GAPDH的大脑含量高于对照组，而在第18天，其水平却相反。结论在EAE小鼠的中央病理过程中，B细胞通过除产生抗体以外的机制发挥作用，并且脑TPI和GADPH的水平与自身免疫诱导损伤的严重程度有关。