Chemoenzymatic Semisynthesis of Proteins,Chemical Reviews

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Chemoenzymatic Semisynthesis of Proteins
Chemical Reviews ( IF 51.4 ) Pub Date : 2019-11-27 , DOI: 10.1021/acs.chemrev.9b00450
Robert E Thompson ₁ , Tom W Muir ₁

Affiliation

Protein semisynthesis—defined herein as the assembly of a protein from a combination of synthetic and recombinant fragments—is a burgeoning field of chemical biology that has impacted many areas in the life sciences. In this review, we provide a comprehensive survey of this area. We begin by discussing the various chemical and enzymatic methods now available for the manufacture of custom proteins containing noncoded elements. This section begins with a discussion of methods that are more chemical in origin and ends with those that employ biocatalysts. We also illustrate the commonalities that exist between these seemingly disparate methods and show how this is allowing for the development of integrated chemoenzymatic methods. This methodology discussion provides the technical foundation for the second part of the review where we cover the great many biological problems that have now been addressed using these tools. Finally, we end the piece with a short discussion on the frontiers of the field and the opportunities available for the future.

中文翻译：

蛋白质的化学酶半合成

蛋白质半合成——本文定义为通过合成和重组片段的组合组装蛋白质——是化学生物学的一个新兴领域，影响了生命科学的许多领域。在这篇评论中，我们对该领域进行了全面的调查。我们首先讨论目前可用于制造含有非编码元素的定制蛋白质的各种化学和酶促方法。本节首先讨论化学来源较多的方法，最后讨论采用生物催化剂的方法。我们还说明了这些看似不同的方法之间存在的共性，并展示了这如何促进集成化学酶方法的发展。这种方法论讨论为审查的第二部分提供了技术基础，其中我们涵盖了现已使用这些工具解决的许多生物学问题。最后，我们通过简短讨论该领域的前沿和未来的机会来结束本文。

更新日期：2019-11-28

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