Conserved motif, gene structure, expression and interaction analysis of C2H2-ZFPs in Brassica rapa, and identified types of genes may play essential roles in flower development, and BrZFP38 was proved to function in flower development by affecting pollen formation. Flower development plays a central role in determining the reproduction of higher plants, and Cys2/His2 zinc-finger proteins (C2H2-ZFPs) widely participate in the transcriptional regulation of flower development. C2H2-ZFPs with various structures are the most widespread DNA-binding transcription factors in plants. In this study, conserved protein motif and gene structures were analyzed to investigate systematically the molecular features of Brassica rapa C2H2-ZFP genes. Expression of B. rapa C2H2-ZFPs in multiple tissues showed that more than half of the family members with different types ZFs were expressed in flowers. The specific expression profiles of these C2H2-ZFPs in different B. rapa floral bud stages were further evaluated to identify their potential roles in flower development. Interaction networks were constructed in B. rapa based on the orthology of flower-related C2H2-ZFP genes in Arabidopsis. The putative cis-regulatory elements in the promoter regions of these C2H2-ZFP genes were thoroughly analyzed to elucidate their transcriptional regulation. Results showed that the orthologs of known-function flower-related C2H2-ZFP genes were conserved and differentiated in B. rapa. A C2H2-ZFP was proved to function in B. rapa flower development. Our study provides a systematic investigation of the molecular characteristics and expression profiles of C2H2-ZFPs in B. rapa and promotes further work in function and transcriptional regulation of flower development.

中文翻译：

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分子表征和表达分析揭示了 Cys2/His2 锌指转录因子在芸苔亚种花发育过程中的作用。中华。

油菜中C2H2-ZFPs的保守基序、基因结构、表达和相互作用分析以及鉴定的基因类型可能在花发育中起重要作用，证明BrZFP38通过影响花粉形成在花发育中起作用。花发育在决定高等植物的繁殖中起核心作用，Cys2/His2锌指蛋白（C2H2-ZFPs）广泛参与花发育的转录调控。具有多种结构的C2H2-ZFPs是植物中最普遍的DNA结合转录因子。在这项研究中，分析了保守的蛋白质基序和基因结构，以系统地研究芸苔 C2H2-ZFP 基因的分子特征。B 的表达 多个组织中的rapa C2H2-ZFPs表明，具有不同类型ZFs的家族成员中有一半以上在花中表达。进一步评估了这些 C2H2-ZFP 在不同 B. rapa 花芽阶段的特定表达谱，以确定它们在花发育中的潜在作用。基于拟南芥中与花相关的C2H2-ZFP基因的直系同源性，在油菜中构建了相互作用网络。对这些 C2H2-ZFP 基因启动子区域中推定的顺式调控元件进行了彻底分析，以阐明它们的转录调控。结果表明，已知功能花相关C2H2-ZFP基因的直系同源物在油菜中是保守和分化的。C2H2-ZFP 被证明在油菜花发育中起作用。