The integral parts of the cell membranes are the functional proteins, which are crucial for cell life. Among them, proton-pumping ATPase and aquaporins appear to be of particular importance. There is some knowledge about the effect of the temperature during plant growth, including stress-inducing temperatures, on the accumulation of the membrane proteins: plasma membrane H+-ATPase and aquaporins, but not much is known about the effect of the phytohormones (i.e. brassinosteroids (BR)) on control of accumulation of these proteins. The aim of our study was to answer the question of how a BR deficit and disturbances in the BR perception/signalling affect the accumulation of plasma membrane H+-ATPase (PM H+-ATPase), the aquaporin HvPIP1 transcript and protein in barley growing at 20 °C and during its acclimation at 5 °C and 27 °C. For the studies, the BR-deficient mutant 522DK (derived from the wild-type Delisa), the BR-deficient mutant BW084 and the BR-signalling mutant BW312 and their wild-type Bowman were used. Generally, temperature of growth was significant factor influencing on the level of the accumulation of the H+-ATPase and HvPIP1 transcript and the PM H+-ATPase and HvPIP1 protein in barley leaves. The level of the accumulation of the HvPIP1 transcript decreased at 5 °C (compared to 20 °C), but was higher at 27 °C than at 20 °C in the analyzed cultivars. In both cultivars the protein HvPIP1 was accumulated in the highest amounts at 27 °C. On the other hand, the barley mutants with a BR deficiency or with BR signalling disturbances were characterised by an altered accumulation level of PM H+-ATPase, the aquaporin HvPIP1 transcript and protein (compared to the wild types), which may suggest the involvement of brassinosteroids in regulating PM H+-ATPase and aquaporin HvPIP1 at the transcriptional and translational levels.

中文翻译：

---

适应高温和低温的大麦油菜素类固醇突变体的质膜ATP酶和水通道蛋白HvPIP1

细胞膜的组成部分是功能性蛋白质，它们对细胞生命至关重要。其中，质子泵送 ATP 酶和水通道蛋白显得尤为重要。有一些关于植物生长过程中温度影响的知识，包括诱导压力的温度，对膜蛋白积累的影响：质膜 H+-ATPase 和水通道蛋白，但对植物激素（即油菜素类固醇）的影响知之甚少(BR)) 控制这些蛋白质的积累。我们研究的目的是回答 BR 缺陷和 BR 感知/信号传导的干扰如何影响质膜 H+-ATP 酶（PM H+-ATP 酶）、水通道蛋白 HvPIP1 转录物和蛋白质在 20 年生长的大麦中的积累的问题。 °C 和在 5 °C 和 27 °C 下的驯化过程中。对于研究，使用 BR 缺陷突变体 522DK（源自野生型 Delisa）、BR 缺陷突变体 BW084 和 BR 信号突变体 BW312 及其野生型 Bowman。一般来说，生长温度是影响大麦叶片中H+-ATPase和HvPIP1转录物以及PM H+-ATPase和HvPIP1蛋白积累水平的重要因素。HvPIP1 转录物的积累水平在 5 °C 时下降（与 20 °C 相比），但在分析的品种中，27 °C 时高于 20 °C。在这两个品种中，蛋白质 HvPIP1 在 27 °C 时积累的量最高。另一方面，具有 BR 缺陷或 BR 信号干扰的大麦突变体的特征是 PM H+-ATPase、水通道蛋白 HvPIP1 转录物和蛋白质（与野生型相比）的积累水平改变，