BACKGROUND Environmental hygiene is one of the most important strategies to prevent hospital-acquired infections by reducing pathogens in haematopoietic cell transplant (HCT) patient rooms. This study was designed in response to JACIE requirements for microbiological monitoring, and aimed to assess environmental hygiene in protective isolation rooms. METHODS Environmental cleanliness was assessed by measuring microbial loads in at-rest and operational conditions sampled from target surfaces, and in passive and active air from rooms occupied by patients with different grades of neutropenia. The study also evaluated whether microbial loads were influenced by isolation precautions. RESULTS The failure rate of cleanliness on target surfaces in at-rest conditions was 0% compared with 37% for surfaces and 13% for passive and active air samples in operational conditions. Differences in failure rates were observed in the rooms of patients with different levels of neutropenia (P=0.036 for surfaces, 0.028% for passive air). No relationship was found between infections and microbial loads. CONCLUSIONS Microbiological assessment integrated with an enhanced monitoring programme for hospital hygiene provides invaluable information to drive infection control policies in HCT patients. These results highlight the need to set and validate strict standards for the assessment of cleanliness in a clinical setting.

中文翻译：

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造血细胞移植患者病房环境清洁度的微生物学评估：实施JACIE标准。

背景技术环境卫生是通过减少造血细胞移植（HCT）患者房间中的病原体来预防医院获得性感染的最重要策略之一。这项研究是针对JACIE对微生物监测的要求而设计的，旨在评估防护隔离室中的环境卫生。方法通过测量静止和操作条件下从目标表面采样的微生物负荷以及不同中性粒细胞减少症患者所居住房间的被动和主动空气中的微生物负荷来评估环境清洁度。该研究还评估了隔离措施是否影响了微生物负荷。结果在静止条件下，目标表面清洁的不合格率为0％，而在操作条件下，目标表面的清洁的不合格率为37％，被动和主动空气样品的清洁率为13％。在中性粒细胞减少症水平不同的患者房间中观察到失败率的差异（表面P = 0.036，被动空气P0.02.8％）。在感染与微生物负荷之间未发现任何关系。结论微生物评估与医院卫生监控程序的增强相结合，为推动HCT患者的感染控制策略提供了宝贵的信息。这些结果强调了需要建立和验证用于临床环境清洁度评估的严格标准。表面为036，被动空气为0.028％）。在感染和微生物负荷之间未发现任何关系。结论微生物评估与增强的医院卫生监测程序相结合，为推动HCT患者的感染控制策略提供了宝贵的信息。这些结果强调了需要建立和验证用于临床环境清洁度评估的严格标准。表面为036，被动空气为0.028％）。在感染和微生物负荷之间未发现任何关系。结论微生物评估与增强的医院卫生监测程序相结合，为推动HCT患者的感染控制策略提供了宝贵的信息。这些结果强调了需要建立和验证用于临床环境清洁度评估的严格标准。