To overcome the deleterious effects of hydrogen peroxide, Lactobacillus plantarum elicits an adaptive response to oxidative stress. In this study, global transcriptomic analysis revealed that L. plantarum CAUH2 expanded its carbon source utilizing profile and enhanced glycolysis to produce more ATP to confront with H2O2 stress. Some antioxidant enzymes including NADH peroxidase, thioredoxin reductase and glutathione peroxidase were 6.11, 36.76 and 6.23-fold up-regulated at transcription level for H2O2 scavenging. Meanwhile, free ferrous iron (Fe2+) was maintained at low concentrations in the cytoplasm, which could limit Fenton reaction and reduce the production of hydroxyl radicals. To repair DNA lesion caused by H2O2, both base excision repair system and recombinational DNA repair pathway were employed by L. plantarum CAUH2. In addition, the expression of methionine sulfoxide reductases and thioredoxin were up-regulated to repair oxidized proteins. It is noteworthy that some transcriptional regulators (Spx, CcpA and MarR1) were predicted to participate in the adaptive response to H2O2 stress, suggesting that L. plantarum CAUH2 utilized a wide array of sensors to monitor oxidative stress and modulated the transcriptional regulation network under H2O2 stress. These findings provide novel insight into the protective mechanisms developed by L. plantarum to cope with oxidative stress.

中文翻译：

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植物乳杆菌CAUH2响应过氧化氢胁迫的全球转录组学分析。

为了克服过氧化氢的有害作用，植物乳杆菌引发对氧化应激的适应性反应。在这项研究中，全球转录组分析显示，植物乳杆菌CAUH2利用分布图扩展了碳源，并增强了糖酵解作用以产生更多的ATP来应对H2O2胁迫。包括NADH过氧化物酶，硫氧还蛋白还原酶和谷胱甘肽过氧化物酶在内的一些抗氧化酶在转录水平上被H2O2清除清除了6.11、36.76和6.23倍。同时，游离亚铁（Fe2 +）在细胞质中保持较低浓度，这可能会限制Fenton反应并减少羟基自由基的产生。为了修复H2O2引起的DNA损伤，植物乳杆菌CAUH2采用了碱基切除修复系统和重组DNA修复途径。此外，蛋氨酸亚砜还原酶和硫氧还蛋白的表达上调以修复氧化蛋白。值得注意的是，一些转录调节因子（Spx，CcpA和MarR1）预计会参与对H2O2胁迫的适应性反应，这表明植物乳杆菌CAUH2利用各种各样的传感器来监测氧化应激并在H2O2下调节转录调控网络。压力。这些发现为植物乳杆菌为应对氧化应激而开发的保护机制提供了新颖的见解。plantarum CAUH2利用各种各样的传感器来监测氧化应激并在H2O2胁迫下调节转录调控网络。这些发现为植物乳杆菌为应对氧化应激而开发的保护机制提供了新颖的见解。plantarum CAUH2利用各种各样的传感器来监测氧化应激并在H2O2胁迫下调节转录调控网络。这些发现为植物乳杆菌为应对氧化应激而开发的保护机制提供了新颖的见解。