Citrus limetta peels (CLP), a waste material generated by juice industries, has scarcely been reported for the production of yeast enzymes. The study was conducted to obtain a multienzyme preparation from a yeast consortium under solid-state fermentation of CLP. The substrate, CLP, was pretreated using either acid or alkali, and factors affecting production of multienzyme were studied by generating two separate Plackett–Burman designs. Since, alkali-pretreated CLP yielded higher titers; therefore, significant factors affecting multienzyme preparation using this substrate were optimized by employing Box–Behnken design. The analysis revealed that under optimized conditions, i.e., cultivation of yeast strains for 72 h to alkali-pretreated CLP moistened with mineral salt medium having pH 5 yielded more than 10 IU mL⁻¹ of cellulase, xylanase, and amylase. The multienzyme was studied for its application to saccharify fruit and non-fruit wastes and for orange juice clarification. The data showed that the enzyme preparation could release 3.03 mg L⁻¹ h⁻¹ of reducing sugars from various crude substrates and was able to reduce turbidity of orange juice by 11% with substantial decrease in viscosity and acidity. Hence, CLP appeared as a promising substrate to produce multienzyme preparation from yeast consortium.

中文翻译：

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柑桔皮：从酵母菌群生产多酶制剂的有前途的基质

鲜有柑桔皮（CLP），它是由果汁工业产生的一种废物，几乎没有用于生产酵母酶的报道。进行该研究以在CLP固态发酵条件下从酵母菌财团获得多酶制剂。使用酸或碱对底物CLP进行了预处理，并通过生成两个单独的Plackett-Burman设计研究了影响多酶生产的因素。由于用碱预处理的CLP的滴定度更高；因此，采用Box–Behnken设计优化了影响使用该底物制备多酶的重要因素。分析表明，在最佳条件下，即将酵母菌株培养72小时，再用pH 5的无机盐培养基浸湿的碱预处理的CLP，其产量超过10 IU mL。纤维素酶，木聚糖酶和淀粉酶的^-1。研究了该多酶在糖化水果和非水果废物以及澄清橙汁中的应用。数据表明，该酶制剂可以从各种粗底物中释放3.03 mg L ^-1  h ^-1的还原糖，并且能够将橙汁的浊度降低11％，同时粘度和酸度显着降低。因此，CLP似乎是从酵母菌体生产多酶制剂的有前途的底物。