Background

It is becoming imperative to develop renewable fuels such as biodiesel which are sustainable and environmentally friendly. Exploiting non-edible oils is more necessary to reduce dependency of edible oils for biodiesel production. The current study investigated biodiesel production from non-edible Salvadora persica seed oil (SPSO) and crude coconut oil (CCO) by Burkholderia cepacia lipase acting as a biocatalyst in a solvent-free system. The biodiesel yield produced from these feedstocks was compared and the effect of ethanol (acyl acceptor) vs. SPSO and CCO in various ratios on biodiesel production was determined.

Results

The presence of medium-chain fatty acids in majority was confirmed for SPSO and CCO while the average molecular weight was calculated as 749.53 g/mol and 664.57 g/mol, respectively. Thin Layer Chromatography indicated ethyl esters in the produced Salvadora and coconut biodiesel samples. Maximum biodiesel yield (around 70%) was obtained at 1:4 oil-to-ethanol molar ratio from both oils followed by a decline at higher ratios. The gas chromatographic analysis of Salvadora biodiesel at 1:4 molar ratio showed that the yield of individual esters was mostly of medium- and long-chain fatty acids. The analysis of coconut biodiesel at 1:4 molar ratio revealed that it consists mainly of the esters of medium-chain fatty acids. A comparison of estimated properties of biodiesel from both the parent oils with the international standard showed that it meets most of the requirements.

Conclusion

The study paves the way for a green route for biodiesel production and would promote the use of non-edible vegetable oils over edible ones to produce biodiesel. Further, it is a right step to use lipases in biodiesel production as compared to chemical catalysts. Ethanol, which can also be produced from biomass fermentation, can be used as acyl acceptor to produce biodiesel and this makes the process eco-friendly. Moreover, Burkholderia cepacia lipase is a good choice among lipases to get high biodiesel yields successfully from SPSO and CCO at low oil-to-ethanol molar ratios.

中文翻译：

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在无溶剂系统中通过非食用Salvadora persica（Pilu）油和粗椰子油的酶促酯交换生产生物柴油

背景

发展可持续的和环境友好的生物燃料等可再生燃料变得势在必行。开发非食用油对于减少食用油对生物柴油生产的依赖性更为必要。当前的研究调查了伯克霍尔德酒原脂肪酶在无溶剂体系中由非食用Salvadora persica种子油（SPSO）和粗椰子油（CCO）生产生物柴油的能力。比较了由这些原料产生的生物柴油产量，并确定了乙醇（酰基受体）与SPSO和CCO的不同比例对生物柴油生产的影响。

结果

对于SPSO和CCO，确认了大多数存在中链脂肪酸，而平均分子量分别计算为749.53 g / mol和664.57 g / mol。薄层色谱法表明所生产的Salvadora和椰子生物柴油样品中存在乙酯。从两种油中以1：4的油与乙醇摩尔比获得最大的生物柴油收率（约70％），然后以较高的比例下降。Salvadora的气相色谱分析摩尔比为1：4的生物柴油表明，单个酯的产率主要为中链和长链脂肪酸。椰子生物柴油在1：4摩尔比下的分析表明，它主要由中链脂肪酸的酯组成。将两种母油中的生物柴油的估计特性与国际标准进行比较，结果表明该柴油符合大多数要求。

结论

该研究为生物柴油生产的绿色道路铺平了道路，并将促进使用非食用植物油而非食用植物油来生产生物柴油。此外，与化学催化剂相比，在生物柴油生产中使用脂肪酶是正确的步骤。乙醇也可以由生物质发酵产生，可以用作生产生物柴油的酰基受体，这使该过程变得环保。此外，伯克霍尔德氏菌洋葱脂肪酶是脂肪酶中的一个不错的选择，可以在低的油/乙醇摩尔比下从SPSO和CCO成功获得高生物柴油收率。