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Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase
Bioresources and Bioprocessing ( IF 4.3 ) Pub Date : 2019-08-07 , DOI: 10.1186/s40643-019-0263-7 Tongjing Sun , Baoqi Zhang , Jinping Lin , Yuhong Ren
中文翻译:
通过将光敏结构域插入氧化酶来对氧化酶活性进行可逆的光控制
更新日期:2019-08-07
Bioresources and Bioprocessing ( IF 4.3 ) Pub Date : 2019-08-07 , DOI: 10.1186/s40643-019-0263-7 Tongjing Sun , Baoqi Zhang , Jinping Lin , Yuhong Ren
Background
Photocontrol of protein activity has become a helpful strategy for regulating biological pathways. Herein, a method for the precise and reversible photocontrol of oxidase activity was developed by using the conformational change of the AsLOV2 domain.Results
The AsLOV2 domain was inserted into the nonconserved sites exposed on the surface of the AdhP protein, and the alov9 fusion was successfully screened for subsequent optical experiments under the assumption that neither of these actions affected the original activity of AdhP protein. The activity of alov9 was noticeably inhibited when the fusion was exposed to 470 nm blue light and recovered within 30 min. As a result, we could precisely and reversibly photocontrol alov9 activity through the optimization of several parameters, including cofactor concentration, light intensity, and illumination time.Conclusions
An efficient method was developed for the photoinhibition of enzymatic activity based on the insertion of the light-sensitive AsLOV2 domain, providing new ideas for photocontrolling metabolic pathways without carriers in the future.中文翻译:
通过将光敏结构域插入氧化酶来对氧化酶活性进行可逆的光控制