Microalgal biofilm-based technologies are of strong interest due to their high biomass concentrations and ability to remove nutrients from wastewater, utilize CO₂ and produce secondary valuable products. This study investigated the biomass production and nutrient removal efficiency of the microalgae Scenedesmus vacuolatus ACUF_053 and Chlorella vulgaris ACUF_809 from a synthetic wastewater, describing a start-up process in a new biofilm photobioreactor (PBR) configuration. Two sets of experiments were performed. The first one compared the performance of a suspended and attached cell system under batch conditions. The second set of experiments was addressed under semi-batch conditions to study the microalgae biofilm development in the PBR. Five stages in the development of the biofilm were identified for S. vacuolatus: attachment, biofilm formation, maturation I, adaptation and maturation II. The biofilm development phases had a different nutrient removal efficiency. S. vacuolatus biofilm showed a higher phosphate demand during the first attachment and formation phases, while it had a higher nitrate demand during the subsequent phases. C. vulgaris biofilm formation was affected by the pH increase (up to 10.6). The biofilm PBR design using both S. vacuolatus and C. vulgaris showed potential for wastewater treatment due the higher nutrient removal rates.

中文翻译：

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使用Scendesmus vacuumlatus和Chlorella vulgaris生物膜启动营养去除系统的启动

基于微藻生物膜的技术因其高生物质浓度以及从废水中去除营养物，利用CO ₂和生产次要有价值的产品的能力而备受关注。这项研究调查了生物质的生产和微藻的营养物的去除效率栅藻vacuolatus ACUF_053和小球藻来自合成废水的ACUF_809，描述了新的生物膜光生物反应器（PBR）配置中的启动过程。进行了两组实验。第一个比较了在批处理条件下悬挂式和附着式电池系统的性能。在半间歇条件下进行第二组实验，以研究PBR中微藻生物膜的发育。针对空腹链球菌鉴定了生物膜发育的五个阶段：附着，生物膜形成，成熟I，适应和成熟II。生物膜发育阶段具有不同的营养去除效率。空泡链球菌生物膜在最初的附着和形成阶段显示出较高的磷酸盐需求，而在随后的阶段显示出较高的硝酸盐需求。普通衣藻的生物膜形成受到pH值升高（最高10.6）的影响。由于使用了更高的养分去除率，同时使用了空肠链球菌和寻常梭状芽胞杆菌的生物膜PBR设计显示出了废水处理的潜力。