Young barley leaf extract (YBL) contains beneficial substances such as fructans, minerals, and vitamins. The effects of YBL administration on the human colonic microbiota and its production of metabolites were evaluated using an in vitro model culture system. Fermentations were started by inoculating fecal samples from nine healthy subjects, with or without 1.5% YBL. Bacterial 16S rRNA sequencing results confirmed that YBL administration significantly increased the relative abundances of bacteria related to the genus Bifidobacterium (p = 0.001, paired t-test) and those of the genera Faecalibacterium, Roseburia, Unclassified Ruminococcaceae, and Lachnospira (p = 0.013, p = 0.019, p = 0.028, and p = 0.034, respectively, paired t-test). Increased abundances of the latter genera corresponded to increased butyrate production in human colonic microbiota models following fermentation with 1.5% YBL, when compared to fermentation without 1.5% YBL (p = 0.006, Dunnett’s test). In addition, YBL administration significantly increased the production levels of amino acids such as lysine, glutamate, serine, threonine, alanine, isoleucine, leucine, valine, and phenylalanine. Therefore, our results showed the health-promoting bifidogenic and butyrogenic effects of YBL.

中文翻译：

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幼叶提取物在体外人结肠微生物群模型中的双歧和丁酸作用


大麦幼叶提取物 (YBL) 含有果聚糖、矿物质和维生素等有益物质。使用体外模型培养系统评估 YBL 给药对人类结肠微生物群及其代谢物产生的影响。通过接种九名健康受试者的粪便样本（含或不含 1.5% YBL）开始发酵。细菌 16S rRNA 测序结果证实，YBL 给药显着增加了双歧杆菌属（ p = 0.001，配对 t 检验）以及粪杆菌属、罗斯伯利亚属、未分类瘤胃球菌科和毛螺菌属相关细菌的相对丰度（ p = 0.013，分别为p = 0.019、 p = 0.028 和p = 0.034，配对 t 检验）。与没有 1.5% YBL 的发酵相比，在用 1.5% YBL 发酵后，后一属丰度的增加对应于人类结肠微生物群模型中丁酸盐产量的增加（ p = 0.006，Dunnett 检验）。此外，施用YBL显着增加赖氨酸、谷氨酸、丝氨酸、苏氨酸、丙氨酸、异亮氨酸、亮氨酸、缬氨酸和苯丙氨酸等氨基酸的产生水平。因此，我们的结果显示了 YBL 具有促进健康的双歧杆菌和丁酸作用。