Sepsis is a serious clinical condition resulting from severe infection. High rates of mortality and tissue damage have been reported in intensive care unit (ICU) patients with sepsis. Bovine lactoferrin (BLF) is a well-known 80-kDa glycoprotein in the transferrin family that inhibits sepsis in low-birth-weight neonates. The present study investigated the protective effects of BLF in a rat model of sepsis-induced acute lung injury (ALI). The wet/dry ratio, lipid peroxidation, antioxidant markers, total protein, total cell count, inflammatory markers and myeloperoxidase (MPO) levels were assessed. Histopathological analysis was also carried out. BLF treatment reduced the wet/dry ratio of lung tissue by 30.7% and 61.3%, and lipid peroxidation by 22.3% and 67%, at concentrations of 100 and 200 mg/kg, respectively. Superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (Gpx) and catalase were increased by more than 50% under treatment with 200 mg/kg BLF. Inflammatory markers, neutrophils, lymphocytes and total cell count were reduced by more than 50% under treatment with 200 mg/kg BLF. BLF treatment significantly reduced MPO activity, by 28.2% and 74.3%, at concentrations of 100 and 200 mg/kg, respectively. Neutrophilic infiltration and edema were observed in control rats. However, BLF treatment restored intestinal microvilli to the normal range and reduced inflammatory cell invasion. Collectively, these results suggest that BLF is an effective therapeutic agent against sepsis-induced ALI.

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牛乳铁蛋白作为新型治疗剂在脓毒症诱发的急性肺损伤大鼠模型中的作用

败血症是由严重感染引起的严重临床状况。据报道，重症监护病房（ICU）脓毒症患者的死亡率和组织损伤率很高。牛乳铁蛋白（BLF）是转铁蛋白家族中众所周知的80 kDa糖蛋白，可抑制低出生体重新生儿的败血症。本研究调查了BLF在脓毒症诱发的急性肺损伤（ALI）大鼠模型中的保护作用。评估干/湿比，脂质过氧化，抗氧化剂标志物，总蛋白，总细胞数，炎症标志物和髓过氧化物酶（MPO）水平。还进行了组织病理学分析。在100和200 mg / kg的浓度下，BLF处理分别使肺组织的湿/干比降低了30.7％和61.3％，脂质过氧化作用降低了22.3％和67％。超氧化物歧化酶（SOD），在200 mg / kg BLF的作用下，谷胱甘肽（GSH），谷胱甘肽过氧化物酶（Gpx）和过氧化氢酶的含量降低了50％以上。用200 mg / kg BLF治疗后，炎症标志物，中性粒细胞，淋巴细胞和总细胞数减少了50％以上。在100和200 mg / kg的浓度下，BLF处理分别显着降低MPO活性，分别降低28.2％和74.3％。在对照大鼠中观察到嗜中性浸润和水肿。但是，BLF治疗可将肠道微绒毛恢复到正常范围，并减少炎症细胞的侵袭。总的来说，这些结果表明BLF是针对败血症诱导的ALI的有效治疗剂。用200 mg / kg BLF治疗后，淋巴细胞和总细胞数减少了50％以上。在100和200 mg / kg的浓度下，BLF处理分别显着降低MPO活性，分别降低28.2％和74.3％。在对照大鼠中观察到嗜中性浸润和水肿。但是，BLF治疗可将肠道微绒毛恢复到正常范围，并减少炎症细胞的侵袭。总的来说，这些结果表明BLF是针对败血症诱导的ALI的有效治疗剂。用200 mg / kg BLF治疗后，淋巴细胞和总细胞数减少了50％以上。在100和200 mg / kg的浓度下，BLF处理分别显着降低MPO活性，分别降低28.2％和74.3％。在对照大鼠中观察到嗜中性浸润和水肿。但是，BLF治疗可将肠道微绒毛恢复到正常范围，并减少炎症细胞的侵袭。总的来说，这些结果表明BLF是针对败血症诱导的ALI的有效治疗剂。BLF治疗可将肠道微绒毛恢复到正常范围，并减少炎症细胞的侵袭。总的来说，这些结果表明BLF是针对败血症诱导的ALI的有效治疗剂。BLF治疗可将肠道微绒毛恢复到正常范围，并减少炎症细胞的侵袭。总的来说，这些结果表明BLF是针对败血症诱导的ALI的有效治疗剂。