Based on several adverse reports of pesticides on reproductive efficiency of various organisms, studies on “reproductive toxicity” have gained importance. Fecundity, reflecting reproductive success of any organism, is governed by several factors from female and male reproductive systems. This present study explored morphological and biochemical alterations in the male reproductive system of a non-target model organism, Drosophila melanogaster following chronic sub-lethal exposure (1^st instar larvae differentially exposed to 1-6 μg/mL until adulthood) to the organophosphate (OP) pesticide, acephate (chronic LC₅₀ 8.71 μg/mL). This study demonstrates altered testis structure, decreased germ cell viability and gross body weight, increased activities of oxidative stress marker lipid peroxidase (LPO), and the endogenous antioxidant enzyme catalase (CAT)in addition with altered expression of reproductive marker proteins like vitellogenin and mitoferrin in acephate-exposed flies when compared to control counterparts. Altered reproductive behavior, indicated by a significant decline in the number of mating pairs, validates the adverse effect of chronic acephate exposure on male reproduction in the non-target insect model D. melanogaster.

基于农药对各种生物繁殖效率的若干不利报道，“生殖毒性”的研究变得越来越重要。生殖力反映了任何生物体的生殖成功，受女性和男性生殖系统的几个因素支配。本研究中在非靶生物模型的男性生殖系统探索形态学和生物化学变化，黑腹果蝇以下慢性亚致死曝光（1只^ST龄幼虫差异暴露于1-6微克/毫升，直到成年期），以有机磷酸酯（ OP）农药，乙酰甲酸盐（慢性LC ₅₀8.71μg/ mL）。这项研究表明睾丸结构改变，生殖细胞活力和总体重下降，氧化应激标记脂质过氧化物酶（LPO）和内源性抗氧化酶过氧化氢酶（CAT）的活性增加以及卵黄蛋白原和米托铁蛋白等生殖标记蛋白的表达发生变化与对照组相比，暴露于乙酰甲胺磷的果蝇中的果蝇。繁殖行为的改变（以交配对数的显着减少为标志）证实了在非目标昆虫模型D. melanogaster中长期暴露于乙酰乙酸盐对雄性繁殖的不利影响。