In this work, carboxymethyl chitosan-graft-eleostearic acid (CMCS-g-EA) was synthesized via the amide reaction between the amino groups of carboxymethyl chitosan and the carboxyl group of eleostearic acid, and then mPEG-NH₂ was grafted to CMCS-g-EA to prepare amphiphilic polymers (mPEG-CMCS-g-EA). The chemical structures of the above conjugates were characterized by FT-IR and ¹H NMR. Both CMCS-g-EA and mPEG-CMCS-g-EA based nano-vesicles were prepared by ultrasonic self-assembly method and they exhibited a low critical aggregation concentration (CAC) of 14.97 μg/mL, 16.82 μg/mL, respectively. The spinosad-loaded mPEG-CMCS-g-EA nano-vesicles ([email protected] NVs) were spherical in shape with an average diameter of 502.8 nm and the zeta potential of −25.60 mV. The encapsulation efficiency (EE) and drug loading content (LC) of [email protected] nano-vesicles were 42.00%, 23.07%, respectively. In vitro release revealed that the [email protected] nano-vesicles exhibited a sustained and pH-responsive drug release property, and could significantly enhance the photostability of spinosad. Furthermore, the toxicological tests demonstrated that the [email protected] nano-vesicles could efficiently inhibit the growth and development of Helicoverpa armigera. These results indicated that the [email protected] nano-vesicles were highly potential for the treatment of Helicoverpa armigera.

在这项工作中，通过羧甲基壳聚糖的氨基与硬脂酸的羧基之间的酰胺反应合成了羧甲基壳聚糖-接枝-硬脂酸（CMCS-g-EA），然后将mPEG-NH ₂接枝到CMCS-上。 g-EA制备两亲性聚合物（mPEG-CMCS-g-EA）。上述缀合物的化学结构通过FT-IR和¹表征1 H NMR。CMCS-g-EA和基于mPEG-CMCS-g-EA的纳米囊泡均通过超声自组装方法制备，它们分别显示出14.97μg/ mL，16.82μg/ mL的低临界聚集浓度（CAC）。装满棘突的mPEG-CMCS-g-EA纳米囊泡（[受电子邮件保护] NVs）呈球形，平均直径为502.8 nm，ζ电位为-25.60 mV。[受电子邮件保护的]纳米囊泡的包封效率（EE）和载药量（LC）分别为42.00％，23.07％。体外释放表明，[电子邮件保护的]纳米囊泡表现出持续的和pH响应的药物释放特性，并且可以显着增强多杀菌素的光稳定性。此外，棉铃虫。这些结果表明，[电子邮件保护的]纳米囊泡在棉铃虫的治疗中具有很高的潜力。