Prevalence, virulence, antimicrobial resistance, and molecular characterization of fluoroquinolone resistance of Vibrio parahaemolyticus from different types of food samples in China.,International Journal of Food Microbiology

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Prevalence, virulence, antimicrobial resistance, and molecular characterization of fluoroquinolone resistance of Vibrio parahaemolyticus from different types of food samples in China.
International Journal of Food Microbiology ( IF 5.0 ) Pub Date : 2019-11-28 , DOI: 10.1016/j.ijfoodmicro.2019.108461
Tao Lei ₁ , Fufeng Jiang ₂ , Min He ₃ , Jumei Zhang ₁ , Haiyan Zeng ₁ , Moutong Chen ₁ , Rui Pang ₁ , Shi Wu ₁ , Lei Wei ₁ , Juan Wang ₄ , Yu Ding ₅ , Qingping Wu ₁

Affiliation

Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong Province 510070, China; State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key, Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong Province 510070, China.
Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong Province 510070, China; State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key, Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong Province 510070, China; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi Province 710021, China.
Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong Province 510070, China; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi Province 710021, China; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong Province 510006, China.
College of Food Science, South China Agricultural University, Guangzhou, Guangdong Province 510642, China.
Department of Food Science & Technology, Jinan University, Guangzhou, Guangdong Province 510632, China.

Vibrio parahaemolyticus is the leading cause of foodborne bacterial poisoning in China. The aim of this research is to conduct a study on the prevalence, virulence, and antimicrobial resistance of V. parahaemolyticus from different types of food samples in 12 different cities of China. Since fluoroquinolones are the major choice of treatment for V. parahaemolyticus infections, the genetic basis for fluoroquinolone resistance in V. parahaemolyticus were also investigated. V. parahaemolyticus was detected in 163 of the 784 food samples collected from 12 different cities in China, resulting in a prevalence of 20.79%. The prevalence of V. parahaemolyticus in ready-to-eat (RTE) food (4.96%) was much lower than those of shrimp (32.62%) and fish (22.00%). Virulence gene screening showed that 44 (27.00%) V. parahaemolyticus strains carried at least one virulence gene. Four isolates from shrimp and three isolates from fish contained both the virulence genes tdh and trh. In addition, the trh was firstly detected in one isolate collected from RTE food. All isolates exhibited relatively high resistance rates to ampicillin (82.21%), gentamicin (19.63%), and tetracycline (14.11%), while <10% of strains were resistant to ciprofloxacin (4.91%), levofloxacin (4.91%), and tetracycline (4.29%). Eight fluoroquinolone-resistant V. parahaemolyticus were selected to determine the molecular basis for fluoroquinolone resistance. These eight isolates belonged to three different types according to enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR). A Ser83Ile substitution in GyrA was deteted in seven fluoroquinolone-resistant strains, except V209 which harbored a Ser83Phe substitution in GyrA. Moreover, A Ser85Leu substitution in ParC was found in five isolates (V52, V53, V61, V163, and V209). Plasmid-mediated quinolone resistance (PMQR) genes were detected in all eight fluoroquinolone-resistant V. parahaemolyticus strains. This is the first report of Ser83Phe substitution in GyrA, qnrD and qnrS1 in V. parahaemolyticus. The information generated in this study will provide valuable information for risk assessment of V. parahaemolyticus infections and future control of antibiotic-resistant V. parahaemolyticus species in China.

更新日期：2019-11-28

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