Silencing against the conserved NAC domain of the potato StNAC103 reveals new NAC candidates to repress the suberin associated waxes in phellem,Plant Science

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Silencing against the conserved NAC domain of the potato StNAC103 reveals new NAC candidates to repress the suberin associated waxes in phellem
Plant Science ( IF 4.2 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.plantsci.2019.110360
Marçal Soler ₁ , Roger Verdaguer ₁ , Sandra Fernández-Piñán ₁ , Dolors Company-Arumí ₁ , Pau Boher ₁ , Elsa Góngora-Castillo ₂ , Marc Valls ₃ , Enriqueta Anticó ₄ , Marisa Molinas ₁ , Olga Serra ₁ , Mercè Figueras ₁

Affiliation

Both suberin and its associated waxes contribute to the formation of apoplastic barriers that protect plants from the environment. Some transcription factors have emerged as regulators of the suberization process. The potato StNAC103 gene was reported as a repressor of suberin polyester and suberin-associated waxes deposition because its RNAi-mediated downregulation (StNAC103-RNAi) over-accumulated suberin and associated waxes in the tuber phellem concomitantly with the induction of representative biosynthetic genes. Here, to explore if other genes of the large NAC gene family participate to this repressive function, we extended the silencing to other NAC members by targeting the conserved NAC domain of StNAC103 (StNAC103-RNAi-c). Transcript profile of the StNAC103-RNAi-c phellem indicated that StNAC101 gene was an additional potential target. In comparison with StNAC103-RNAi, the silencing with StNAC103-RNAi-c construct resulted in a similar effect in suberin but yielded an increased load of associated waxes in tuber phellem, mainly alkanes and feruloyl esters. Globally, the chemical effects in both silenced lines are supported by the transcript accumulation profile of genes involved in the biosynthesis, transport and regulation of apoplastic lipids. In contrast, the genes of polyamine biosynthesis were downregulated. Altogether these results point out to StNAC101 as a candidate to repress the suberin-associated waxes.

中文翻译：

对马铃薯 StNAC103 保守 NAC 域的沉默揭示了新的 NAC 候选物抑制木栓中与木栓质相关的蜡

木栓质及其相关蜡都有助于形成保护植物免受环境影响的质外体屏障。一些转录因子已经成为木栓化过程的调节剂。据报道，马铃薯 StNAC103 基因是木栓质聚酯和木栓质相关蜡沉积的阻遏物，因为其 RNAi 介导的下调 (StNAC103-RNAi) 在块茎中过度积累木栓质和相关蜡，同时诱导代表性生物合成基因。在这里，为了探索大型 NAC 基因家族的其他基因是否参与这种抑制功能，我们通过靶向 StNAC103 的保守 NAC 结构域（StNAC103-RNAi-c）将沉默扩展到其他 NAC 成员。StNAC103-RNAi-c phellem 的转录谱表明 StNAC101 基因是一个额外的潜在目标。与 StNAC103-RNAi 相比，StNAC103-RNAi-c 构建体的沉默在木栓质中产生了类似的效果，但在块茎木栓中产生了相关蜡的负载增加，主要是烷烃和阿魏酸酯。在全球范围内，参与质外体脂质的生物合成、运输和调节的基因的转录积累谱支持了这两个沉默品系中的化学效应。相比之下，多胺生物合成的基因被下调。总而言之，这些结果表明 StNAC101 是抑制木栓质相关蜡的候选物。参与质外体脂质的生物合成、运输和调节的基因的转录积累谱支持了两种沉默品系中的化学作用。相比之下，多胺生物合成的基因被下调。总而言之，这些结果表明 StNAC101 是抑制木栓质相关蜡的候选物。参与质外体脂质的生物合成、运输和调节的基因的转录积累谱支持了两种沉默品系中的化学效应。相比之下，多胺生物合成的基因被下调。总之，这些结果表明 StNAC101 是抑制与木栓质相关蜡的候选物。

更新日期：2020-02-01

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