Light modulates almost every aspect of plant physiology, including plant-pathogen interactions. Among these, the hypersensitive response (HR) of plants to pathogens is characterized by a rapid and localized programmed cell death (PCD), which is critical to restrict the spread of pathogens from the infection site. The aim of this work was to study the role of light in the interaction between Pseudomonas syringae pv. tomato DC3000 (Pto DC3000) and non-host tobacco plants. To this end, we examined the HR under different light treatments (white and red light) by using a range of well-established markers of PCD. The alterations found at the cellular level included: i) loss of membrane integrity and nuclei, ii) RuBisCo and DNA degradation, and iii) changes in nuclease profiles and accumulation of cysteine proteinases. Our results suggest that red light plays a role during the HR of tobacco plants to Pto DC3000 infection, delaying the PCD process.

中文翻译：

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红光延迟了番茄丁香假单胞菌 pv 番茄 DC3000 和烟草植物之间非宿主相互作用中的程序性细胞死亡


光几乎调节植物生理学的各个方面，包括植物与病原体的相互作用。其中，植物对病原体的过敏反应（HR）的特点是快速且局部的程序性细胞死亡（PCD），这对于限制病原体从感染部位传播至关重要。这项工作的目的是研究光在丁香假单胞菌之间相互作用中的作用。番茄 DC3000 (Pto DC3000) 和非寄主烟草植物。为此，我们使用一系列成熟的 PCD 标记物检查了不同光处理（白光和红光）下的 HR。在细胞水平上发现的改变包括：i) 膜完整性和细胞核的丧失，ii) RuBisCo 和 DNA 降解，以及 iii) 核酸酶谱的变化和半胱氨酸蛋白酶的积累。我们的结果表明，红光在烟草植物对 Pto DC3000 感染的 HR 过程中发挥了作用，延迟了 PCD 过程。