The generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish technologies for the expression, isolation and characterization of IMPs. Here we summarize some of the key aspects, which are important to support structure-based drug design (SBDD) in drug discovery projects. We furthermore include timeline estimates and an overview of the target selection and biophysical screening approaches.

中文翻译：

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针对整体膜蛋白靶标的生化和生物物理套件的开发：综述。

异源系统中整合膜蛋白（IMPs）的产生及其表征仍然是生物医学研究中的主要挑战。在学术界和制药行业都投入了巨大的努力，以建立用于表达，分离和表征IMP的技术。在这里，我们总结了一些关键方面，这些方面对于在药物开发项目中支持基于结构的药物设计（SBDD）至关重要。我们还包括时间轴估计以及目标选择和生物物理筛选方法的概述。